BLASTO: a tool for searching orthologous groups.
BLASTO: a tool for searching orthologous groups.
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Blasto：搜索直系同源组的工具。
DOI：
10.1093/nar/gkm278
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发表时间：
2007-07
影响因子：
14.9
通讯作者：
Landweber LF
中科院分区：
文献类型：
--
作者：
Zhou Y;Landweber LF
我们提出了BLAST上的Orthopathy组（BLASTO），一个修改后的BLAST工具搜索Orthopathy组数据。它将每个正交组视为一个单元，并输出正交组的排序列表，而不是单个序列。通过过滤掉冗余和推定的旁系同源物，序列比较的直系亲属组，而不是在数据库中的单个序列，可以提高功能预测和系统发育推断。BLASTO基于直向组中的个体BLAST命中计算每个直向组的显著性得分，使用组中的分类群的数量作为任选的权重。这使用户能够控制物种的物种多样性的正交组。BLASTO整合了NCBI Clusters of Orthopathy Group、NCBI euKaryotic Orthopathy Group、OrthoMCL、MultiParanoid和TIGR等多物种同源基因数据库，为整合同源基因信息进行功能推断和个体序列进化研究提供了一个有用的平台。BLASTO可在http://oxytricha.princeton.edu/BlastO上在线访问。
We present BLAST on Orthologous groups (BLASTO), a modified BLAST tool for searching orthologous group data. It treats each orthologous group as a unit and outputs a ranked list of orthologous groups instead of single sequences. By filtering out redundancy and putative paralogs, sequence comparisons to orthologous groups, instead of to single sequences in the database, can improve both functional prediction and phylogenetic inference. BLASTO computes the significance score of each orthologous group based on the individual BLAST hits in the orthologous group, using the number of taxa in the group as an optional weight. This allows users to control the species diversity of the orthologous groups. BLASTO incorporates the best-known multispecies ortholog databases, including NCBI Clusters of Orthologous Group, NCBI euKaryotic Orthologous Group database, OrthoMCL, MultiParanoid and TIGR Eukaryotic Gene Orthologues database, and offers a useful platform to integrate orthology information into functional inference and evolutionary studies of individual sequences. BLASTO is accessible online at http://oxytricha.princeton.edu/BlastO.