Characterization of drug authenticity using thin-layer chromatography imaging with a mobile phone
Characterization of drug authenticity using thin-layer chromatography imaging with a mobile phone
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DOI：
10.1016/j.jpba.2016.03.018
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发表时间：
2016-06-05
影响因子：
3.4
通讯作者：
Cunningham, Brian T.
中科院分区：
文献类型：
--
作者：
Yu, Hojeong;Le, Huy M.;Cunningham, Brian T.
薄层色谱（TLC）由于其简单和低成本，在化学、生物学和药理学中具有无数的分离应用。虽然用于TLC的台式实验室样品应用和检测系统提供了TLC斑点位置和密度的精确定量，但在许多应用中，廉价和便携式仪器将大大扩展该技术的适用性。在这项工作中，我们使用与普通移动的手机接口的定制3D打印支架，通过TLC演示了药物化合物的身份验证和浓度测定。支架将移动的电话的内部后置摄像头保持在相对于UV灯和TLC板的固定位置，TLC板包括处于固定相的磷光体。因此，对照片的分析揭示了紫外吸收药物的主要斑点的位置和强度。自动化图像分析软件确定暗点的中心位置和密度，其使用已知药物化合物和浓度的积分校准点，可用于确定药物是否已被稀释或替代。已经开发了两种独立的图像处理方法，其可以基于智能电话的处理能力来选择。每种方法都能够辨别5%的药物浓度差异。使用已手动应用的奈韦拉平、阿莫地喹和扑热息痛的单组分溶液，基于移动的手机的检测仪器提供的测量结果与市售基于实验室的台式TLC密度计获得的测量结果相当。(C)© 2016 Elsevier B.V.版权所有。
Thin-layer chromatography (TLC) has a myriad of separation applications in chemistry, biology, and pharmacology due to its simplicity and low cost. While benchtop laboratory sample application and detection systems for TLC provide accurate quantitation of TLC spot positions and densities, there are many applications where inexpensive and portable instruments would greatly expand the applicability of the technology. In this work, we demonstrate identity verification and concentration determination of pharmaceutical compounds via TLC using a custom 3D-printed cradle that interfaces with an ordinary mobile phone. The cradle holds the mobile phone's internal, rear-facing camera in a fixed position relative to a UV lamp and a TLC plate that includes a phosphor in the stationary phase. Analysis of photographs thus reveals the locations and intensities of principal spots of UV-absorbing drugs. Automated image analysis software determines the center location and density of dark spots, which, using integrated calibration spots of known drug compounds and concentrations, can be used to determine if a drug has been diluted or substituted. Two independent image processing approaches have been developed that may be selected based upon the processing capabilities of the smartphone. Each approach is able to discern 5% drug concentration differences. Using single-component solutions of nevirapine, amodiaquine, and paracetamol that have been manually applied, the mobile phone-based detection instrument provides measurements that are equivalent to those obtained with a commercially available lab-based desktop TLC densitometer. (C) 2016 Elsevier B.V. All rights reserved.