Radioimmunological measurement of leptin in plasma of obese and diabetic human subjects
Radioimmunological measurement of leptin in plasma of obese and diabetic human subjects
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DOI：
10.1210/en.137.4.1501
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发表时间：
1996-04-01
期刊：
影响因子：
4.8
通讯作者：
Lang, RE
中科院分区：
文献类型：
--
作者：
McGregor, GP;Desaga, JF;Lang, RE
在小鼠中的研究已经确定ob基因产物瘦素作为调节体重稳态和能量平衡的信号因子。编码蛋白的缺陷生产可能是肥胖症的发展的原因之一。使用高亲和力抗体，在免疫组织化学研究中特异性染色的人脂肪细胞，建立放射免疫测定法，并定量瘦和肥胖的人受试者的血浆中的瘦素免疫反应性。色谱分析表明，血浆中的免疫反应性物质与人脂肪提取物中发现的相同，代表分子大小约为16 kD的蛋白质。在75名瘦型和肥胖型人类受试者（体重指数（BMI）17.7 - 87.3）的血浆中测量空腹水平。瘦受试者（BMI小于或等于28）血浆中瘦素的平均浓度为69.3 +/- 36.9 fmol/ml血浆（平均值+/- SD，n=27）。肥胖者血浆中测得的最高浓度为533.3 fmol/ml。结果表明，血清中的总胆固醇水平与BMI呈显著正相关（r=0.77，p
Studies in mice have identified the ob gene product, leptin, as a signaling factor regulating body weight homeostasis and energy balance. Defective production of the encoded protein may be one of the causes for the development of obesity. Using a high affinity antibody, that in immunohistochemical studies specifically stained human adipocytes, a radioimmunoassay was established and leptin immunoreactivity was quantified in plasma of lean and obese human subjects. Chromatographic analysis suggested that the immunoreactive material in plasma is identical to that found in extracts from human fat and represents a protein with a molecular size of approximately 16 kD. Fasting levels were measured in plasma of 75 lean and obese human subjects (body mass index (BMI) 17.7 - 87.3). The mean concentration of leptin in plasma of lean subjects (BMI less than or equal to 28) was 69.3 +/- 36.9 fmol/ml plasma (mean +/- SD, n=27). The highest concentration measured in obese was 533.3 fmol/ml plasma. The levels showed a strong positive correlation with BMI (r=0.77, p