Dictyostelium discoideum mRNAs developmentally regulated during spore germination have short half-lives
Dictyostelium discoideum mRNAs developmentally regulated during spore germination have short half-lives
复制标题
盘基网柄菌孢子萌发过程中受发育调节的 mRNA 半衰期较短
DOI：
10.1128/mcb.5.1.133-139.1985
复制
发表时间：
1985
影响因子：
5.3
通讯作者：
H. Ennis
中科院分区：
文献类型：
--
作者：
R. Kelly;L. J. Kelly;H. Ennis
利用先前分离的pLK109、pLK229和pRK270三个cDNA克隆，研究了Dictyoselium disideum孢子萌发过程中mRNA的衰变，这三个克隆对孢子萌发过程中发育调控的mRNA具有特异性。组成mRNA pLK125的半衰期也被确定，它存在于萌发、生长和发育过程中。诺加霉素是一种dna插入化合物，用于抑制RNA合成。加药后每隔一段时间分离总RNA，采用Northern blot和RNA点杂交检测cDNA克隆特异性mrna的衰减情况。如果激活休眠孢子后立即添加诺加霉素，pLK229和pLK109 mRNA均不腐烂，但pLK125 mRNA却腐烂。虽然pLK109 mRNA在这些条件下没有腐烂，但激活后1小时的RNA比休眠孢子中的RNA小，表明其正常加工。在激活后1小时，pLK229-和plk125特异性mrna呈指数衰减，半衰期分别为24、39和165分钟。在相同的条件下，plk109特异性mRNA的衰减是双相的。38% mRNA的半衰期为5.5 min，其余mRNA的半衰期为115 min。似乎诺加霉素抑制了mRNA降解途径中一个不稳定组分的合成，而该组分是pLK109 mRNA降解所需要的。通过外推代表快速衰减成分的曲线，计算出plk109特异性mRNA的半衰期为18分钟。在孢子萌发过程中发育调控的mRNA的半衰期比构成信使的半衰期短，也比之前测定的总双胚轴聚腺苷化mRNA的平均半衰期短3至4小时。
mRNA decay was studied during spore germination in Dictyoselium discoideum by the use of three previously isolated cDNA clones, pLK109, pLK229, and pRK270, which are specific for mRNAs developmentally regulated during spore germination. The half-life of a constitutive mRNA, pLK125, which is present throughout germination, growth, and development, as also determined. Nogalamycin, a DNA-intercalating compound, was used to inhibit RNA synthesis. Total RNA was isolated at intervals after addition of the drug, and the decay of mRNAs specific for the cDNA clones was determined by both Northern blot and RNA dot hybridization. If nogalamycin was added immediately after activation of dormant spores, neither pLK229 nor pLK109 mRNA decayed, but pLK125 mRNA did decay. Although pLK109 mRNA did not decay under these conditions, the RNA was smaller 1 h after activation than in dormant spores, indicating that it was processed normally. At 1 h after activation, pLK229-, pLK125-specific mRNAs decayed exponentially, with half-lives of 24, 39, and 165 min, respectively. Under the same conditions, decay of pLK109-specific mRNA was biphasic. Thirty-eight percent of the mRNA decayed with a half-life of 5.5 min, and the remainder decayed with a half-life of 115 min. It seems likely that nogalamycin inhibits the synthesis of an unstable component of the mRNA degradative pathway which is needed continuously for the decay of pLK109 mRNA. By extrapolating the curve representing the rapidly decaying component, a half-life of 18 min was calculated for pLK109-specific mRNA. The mRNAs developmentally regulated during spore germination have half-lives shorter than that of the constitutive messenger and shorter than the average half-life of 3 to 4 h previously determined for total Dicyostelium polyadenylated mRNA.
黄粘球菌子实体形成过程中 mRNA 寿命较长的证据。
DOI：
10.1073/pnas.80.5.1467
发表时间：
1983
影响因子：
11.1
作者：
Nelson,DR;Zusman,DR
通讯作者：
Zusman,DR
盘基网柄菌中信使 RNA 的半衰期。
影响因子：
2.7
作者：
Casey,L;Palatnik,CM;Jacobson,A
通讯作者：
Jacobson,A