Medium weight neurofilament mRNA in goldfish Mauthner axoplasm.
Medium weight neurofilament mRNA in goldfish Mauthner axoplasm.
复制标题
金鱼 Mauthner 轴浆中的中等重量神经丝 mRNA。
DOI：
10.1016/0304-3940(96)12860-3
复制
发表时间：
1996
影响因子：
2.5
通讯作者：
Bittner,GD
中科院分区：
文献类型：
--
作者：
Weiner,OD;Zorn,AM;Krieg,PA;Bittner,GD
虽然轴突通常被认为缺乏合成蛋白质的能力，但据报道金鱼的Mauthner轴突（M-axon）含有一些翻译机制的基本成分，如转运RNA（tRNA），核糖体RNA（RNNA）和核糖体。为了确定M-轴突是否也含有mRNA，我们分离了无胶质污染的M-轴浆样品，如通过缺乏胶质特异性mRNA和蛋白质所证明的。逆转录-聚合酶链反应（RT-PCR）的M-轴浆cDNA的引物的存在下，金鱼中等重量的神经丝（NF-M）基因产生的预期长度的金鱼NF-M mRNA的RT-PCR扩增的单一产品。该mRNA可能指导M轴浆内NF-M的蛋白质合成。
Although axons are generally considered to lack the ability to synthesize proteins, the Mauthner axon (M-axon) of the goldfish has been reported to contain some of the basic components of the translational machinery, such as transfer RNA (tRNA), ribosomal RNA (rNNA), and ribosomes. To determine if the M-axon also contains mRNA, we isolated samples of M-axoplasm free of glial contamination as demonstrated by the absence of glial-specific mRNA and protein. Reverse transcription-polymerase chain reaction (RT-PCR) of M-axoplasmic cDNA in the presence of primers for the goldfish medium-weight neurofilament (NF-M) gene produced a single product of the expected length for RT-PCR amplification of goldfish NF-M mRNA. This mRNA might direct protein synthesis of NF-M within the M-axoplasm.