Hydrodynamic Modulation of Embryonic Stem Cell Differentiation by Rotary Orbital Suspension Culture
Hydrodynamic Modulation of Embryonic Stem Cell Differentiation by Rotary Orbital Suspension Culture
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DOI：
10.1002/bit.22578
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发表时间：
2010-02-15
影响因子：
3.8
通讯作者：
McDevitt, Todd C.
中科院分区：
文献类型：
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作者：
Sargent, Carolyn Y.;Berguig, Geoffrey Y.;McDevitt, Todd C.
胚胎干细胞（ESCs）可以分化为所有体细胞类型，但指导ESC命运的有效策略的发展取决于能够影响细胞表型的环境参数的定义。ESCs通常通过称为胚状体（EBs）的细胞聚集体分化，但目前的培养方法，如悬垂和静态悬浮，产生相对较少或异质的胚状体群体。另外，旋转轨道悬浮培养可以提高EB形成效率、细胞产量和均匀性，而不会对分化产生不利影响。因此，本研究的目的是系统地研究由旋转轨道振动产生的流体动力条件对EB形成、结构和分化的影响。在旋转轨道速度（20-60转/分）范围内进行悬浮培养的小鼠ESCs由于细胞聚集动力学的差异，表现出不同的EB形成大小和产量。计算流体动力学分析表明，旋转轨道振动产生相对均匀和温和的剪应力(
Embryonic stern cells (ESCs) can differentiate into all somatic cell types, but the development of effective strategies to direct ESC fate is dependent upon defining environmental parameters capable of influencing cell phenotype. ESCs are commonly differentiated via cell aggregates referred to as embryoid bodies (EBs), but Current culture methods, Such as hinging drop and static Suspension, yield relatively few or heterogeneous populations of EBs. Alternatively, rotary orbital suspension culture enhances EB formation efficiency, cell yield, and homogeneity without adversely affecting differentiation. Thus, the objective of this study was to systematically examine the effects of hydrodynamic conditions created by rotary orbital shaking on EB firmation, structure, and differentiation. Mouse ESCs introduced to suspension culture at a range of rotary orbital speeds (20-60 rpm) exhibited variable EB formation sizes and yields due to differences in the kinetics of cell aggregation. Computational fluid dynamic analyses indicated that rotary orbital shaking generated relatively uniform and mild shear stresses (