The molecular and cellular features of 2-cell-like cells: a reference guide
The molecular and cellular features of 2-cell-like cells: a reference guide
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DOI：
10.1242/dev.189688
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发表时间：
2020-08-01
期刊：
影响因子：
4.6
通讯作者：
Torres-Padilla, Maria-Elena
中科院分区：
文献类型：
--
作者：
Genet, Marion;Torres-Padilla, Maria-Elena
目前，两种主要的细胞培养模型主导多能干细胞研究：胚胎干细胞（ESC）和诱导多能干细胞（iPSC）。由于它们能够促进和形成体内所有组织，ESC和iPSC在理解多能状态，早期胚胎发育和细胞分化以及制定再生医学策略方面具有非常宝贵的价值。相对而言，人们对全能性--一种具有更大发育潜力的细胞状态--知之甚少。在小鼠中，只有受精卵和2细胞期胚胎的卵裂球是真正的全能性，因为它们可以单独发育形成胚胎及其所有支持性胚外组织。然而，在小鼠ESC培养物中发现了一种罕见的细胞亚群，具有2-细胞胚胎卵裂球和扩大的细胞命运潜力的特征，提供了一种生物化学上易于处理的模型，使全能性的体外研究成为可能。在这里，我们总结了目前已知的这些2-细胞样细胞（2CLC）的特征，以努力为社区提供参考，并澄清我们所知道的关于他们的身份到目前为止。
Currently, two main cell culture models predominate pluripotent stem cell research: embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Thanks to their ability to contribute to and form all tissues within the body, ESCs and iPSCs have proven invaluable in understanding pluripotent states, early embryonic development and cell differentiation, as well as in devising strategies for regenerative medicine. Comparatively little is known about totipotency - a cellular state with greater developmental potential. In mice, only the zygote and the blastomeres of the 2-cell-stage embryo are truly totipotent, as they alone can develop to form the embryo and all of its supportive extra-embryonic tissues. However, the discovery of a rare subpopulation of cells in murine ESC cultures, possessing features of 2-cell embryo blastomeres and expanded cell fate potential, has provided a biochemically tractable model to enable the in vitro study of totipotency. Here, we summarize current known features of these 2-cell-like cells (2CLCs) in an effort to provide a reference for the community, and to clarify what we know about their identity so far.