MOLECULAR ANALYSIS OF A GENTAMICIN RESISTANCE TRANSPOSON-LIKE ELEMENT ON PLASMIDS ISOLATED FROM NORTH-AMERICAN STAPHYLOCOCCUS-AUREUS STRAINS
MOLECULAR ANALYSIS OF A GENTAMICIN RESISTANCE TRANSPOSON-LIKE ELEMENT ON PLASMIDS ISOLATED FROM NORTH-AMERICAN STAPHYLOCOCCUS-AUREUS STRAINS
复制标题
DOI：
10.1128/aac.34.11.2106
复制
发表时间：
1990-11-01
影响因子：
4.9
通讯作者：
SKURRAY, RA
中科院分区：
文献类型：
--
作者：
BYRNE, ME;GILLESPIE, MT;SKURRAY, RA
在澳大利亚和北美分离的金黄色葡萄球菌菌株中，质粒编码的对氨基糖苷类庆大霉素（Gm）、妥布霉素（Tm）和卡那霉素（Km）（GmTmKmr）的耐药性似乎由一个耐药决定簇介导。在澳大利亚的分离株中，这个决定子的侧翼是一个1.3 kb插入序列IS 256的反向拷贝，从而形成一个复合转座子Tn 4001。两个接合性质粒和一个非接合性质粒的分析。 在北美分离的金黄色葡萄球菌中发现，这些质粒上的GmTmKmr决定簇也侧翼有反向重复序列。在非接合性质粒中，这些重复序列仅包括紧邻GmTmKmr区域的425 bp的IS 256，并且与Tn 4001上的相同。该截短的Tn 4001元件侧接插入元件IS 257的拷贝，并且这些元件一起形成截短的Tn 4001-IS 257杂合转座子样结构。IS 257的第三个拷贝位于距离杂交结构418 bp处。截短的Tn 4001和三个重复的IS 257存在于所研究的质粒上的保守位点。在两个接合质粒上鉴定了另外四个IS 257拷贝。这些元素侧翼的决定因素，耐氨基糖苷类新霉素和巴龙霉素和溴化乙锭和季铵化合物，以及该地区参与接合质粒转移。
Plasmid-encoded resistance to the aminoglycosides gentamicin (Gm), tobramycin (Tm), and kanamycin (Km) (GmTmKmr) in strains of Staphylococcus aureus isolated in Australia and North America appears to be mediated by one resistance determinant. In Australian isolates, this determinant is flanked by inverted copies of a 1.3-kb insertion sequence, IS256, thereby forming a composite transposon, Tn4001. Analysis of two conjugative plasmids and a related nonconjugative plasmid from strains of S. aureus isolated in North America showed that the GmTmKmr determinant on these plasmids is also flanked by inverted repeats. In the nonconjugative plasmid, these repeats include only 425 bp of IS256 immediately adjacent to the GmTmKmr region and identical to that on Tn4001. This truncated Tn4001 element is flanked by copies of the insertion element IS257, and together these elements form a truncated Tn4001-IS257 hybrid transposonlike structure. A third copy of IS257 was located 418 bp from the hybrid structure. The truncated Tn4001 and three repeats of IS257 were present at a conserved site on the plasmids studied. Four additional copies of IS257 were identified on the two conjugative plasmids. These elements flank determinants for resistance to aminoglycosides neomycin and paromomycin and to ethidium bromide and quaternary ammonium compounds, as well as the region involved in conjugative plasmid transfer.