The myoepithelial cell differentiation of mucoepidermoid carcinoma in a collagen gel-based coculture model.
The myoepithelial cell differentiation of mucoepidermoid carcinoma in a collagen gel-based coculture model.
复制标题
基于胶原凝胶的共培养模型中粘液表皮样癌的肌上皮细胞分化。
DOI：
10.1111/j.0904-2512.2004.00056.x
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Jin Kim
中科院分区：
文献类型：
--
作者：
J. Yook;S. Lee;Yong;J. Huh;I. Cha;Jin Kim
背景
BACKGROUND
Mucoepidermoid carcinomas (MECs) are the most common malignant tumor of the salivary glands; however, the histogenesis of MECs has been still controversial. This study was undertaken to investigate the histogenesis of MECs by the examination of their collagen gel-based coculture tissue and transplanted tumors.
METHODS
Two cell lines from a primary and a metastatic MECs were established and characterized by the mutational analysis of the p53 gene and in vivo tumorigenicity in athymic nude mice. Collagen gel-based organotypic cocultures were performed, and the ultrastructural and immunohistochemical findings were examined.
RESULTS
Two cell lines demonstrated p53 point mutation at the same codon. A metastatic cell line of MEC showed in vivo tumorigenicity. Transplanted tumors and the collagen gel-based culture tissues showed poorly differentiated squamous cell carcinomas devoid of mucous cell differentiation; however, they disclosed the differentiation of myoepithelial cells.
CONCLUSIONS
MECs appear to be centered on the squamous cell differentiation, and the specific differentiation of myoepithelial or mucous cells seems to be modulated by the property of microenvironment.