Altered Fecal Microbiome and Metabolome in a Mouse Model of Choroidal Neovascularization.
Altered Fecal Microbiome and Metabolome in a Mouse Model of Choroidal Neovascularization.
复制标题
脉络膜新生血管小鼠模型中粪便微生物组和代谢组的改变。
DOI：
10.3389/fmicb.2021.738796
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发表时间：
2021
影响因子：
5.2
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Li Y;Cai Y;Huang Q;Tan W;Li B;Zhou H;Wang Z;Zou J;Ding C;Jiang B;Yoshida S;Zhou Y
脉络膜新生血管（CNV）是新生血管性年龄相关性黄斑变性（nAMD）的定义特征。肠道菌群可能在nAMD的发病机制中起重要作用。本研究旨在揭示肠道微生物组和粪便代谢组在激光诱导的CNV小鼠模型中的作用。收集有或无激光诱导CNV的C57 BL/6 J小鼠的粪便。多组学分析，包括16 S rRNA基因测序和非靶向代谢组学，进行分析的肠道微生物组成和粪便代谢谱在CNV小鼠的变化。CNV小鼠的肠道微生物群发生了显著改变。CNV组小鼠粪便中的白背飞虱属的丰度显著上调，而对照组中包括Prevotellaceae_NK3B31_group、白背飞虱属_Soleaferrea和Truepera在内的16个属的丰度显著高于CNV组。与对照小鼠相比，粪便代谢组学在CNV小鼠中鉴定出73种改变的代谢物（包括52种强烈显著改变的代谢物）。相关性分析表明，粪便代谢产物的改变与肠道微生物群的属，如毛螺菌科_UCG-001和刺孢属_刺孢菌属之间存在显著相关性。此外，KEGG分析揭示了与这些改变的代谢物相关的六条途径，如ABC转运蛋白、初级胆汁酸生物合成和类固醇激素生物合成途径。该研究在CNV小鼠模型中鉴定了改变的粪便微生物组和代谢组。nAMD的发病机制可能与nAMD的微生物、代谢产物及相关通路的改变有关。
Choroidal neovascularization (CNV) is the defining feature of neovascular age-related macular degeneration (nAMD). Gut microbiota might be deeply involved in the pathogenesis of nAMD. This study aimed to reveal the roles of the gut microbiome and fecal metabolome in a mouse model of laser-induced CNV. The feces of C57BL/6J mice with or without laser-induced CNV were collected. Multi-omics analyses, including 16S rRNA gene sequencing and untargeted metabolomics, were conducted to analyze the changes in the gut microbial composition and the fecal metabolomic profiles in CNV mice. The gut microbiota was significantly altered in CNV mice. The abundance of Candidatus_Saccharimonas was significantly upregulated in the feces of CNV mice, while 16 genera, including Prevotellaceae_NK3B31_group, Candidatus_Soleaferrea, and Truepera, were significantly more abundant in the controls than in the CNV group. Fecal metabolomics identified 73 altered metabolites (including 52 strongly significantly altered metabolites) in CNV mice compared to control mice. Correlation analysis indicated significant correlations between the altered fecal metabolites and gut microbiota genera, such as Lachnospiraceae_UCG-001 and Candidatus_Saccharimonas. Moreover, KEGG analysis revealed six pathways associated with these altered metabolites, such as the ABC transporter, primary bile acid biosynthesis and steroid hormone biosynthesis pathways. The study identified an altered fecal microbiome and metabolome in a CNV mouse model. The altered microbes, metabolites and the involved pathways might be associated with the pathogenesis of nAMD.