Intriguing "chameleon" fluorescent bioprobes for the visualization of lipid droplet-lysosome interplay
Intriguing "chameleon" fluorescent bioprobes for the visualization of lipid droplet-lysosome interplay
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有趣的“变色龙”荧光生物探针用于脂滴-溶酶体相互作用的可视化
DOI：
10.1016/j.biomaterials.2019.03.002
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发表时间：
2019-05-01
期刊：
影响因子：
14
通讯作者：
Tang, Ben Zhong
中科院分区：
文献类型：
--
作者：
Hu, Rong;Chen, Bin;Tang, Ben Zhong
脂滴与溶酶体的相互作用在细胞代谢中起着重要的作用，这一过程的可视化可以为细胞器的通讯和功能提供有用的信息。然而，基于有机荧光团的荧光生物探针可以响应ld -溶酶体的相互作用是非常罕见的。本文通过将一种新的红色荧光分子TPA-WITDO包封在聚合物基质中（DSPE-PEG(2000)），实现了具有高光稳定性、优异生物相容性和细胞内极性敏感性的荧光生物探针。它们可以依次定位在溶酶体和具有红色和青色发射的ld中。通过对发射颜色变化的监测，可视化了探针从溶酶体中逸出，然后在ld中富集，最后在ld消耗后返回溶酶体的有趣动态过程。此外，利用高信噪比的探头实现了ld动态运动和消耗的跟踪。该探针在ld和溶酶体中独特的标记行为和独特的双发射特性使其成为ld溶酶体相关生物过程和代谢疾病的荧光可视化研究的理想工具。
The interplay of lipid droplets (LDs) and lysosome plays an important role in cell metabolism, and the visualization of this process can provide useful information of organelle communication and function. However, fluorescent bioprobes based on organic fluorophores that can respond to LD-lysosome interplay are much rare. Herein, fluorescent bioprobes with high photostability, excellent biocompatibility and intracellular polarity sensitivity are achieved by encapsulating a new red fluorogenic molecule TPA-WITDO within polymeric matrix (DSPE-PEG(2000)). They can sequentially localize in lysosome and LDs with red and cyan emissions, respectively. By monitoring the emission color change, the interesting dynamic processes of the probes escaping from lysosome and then enriching in LDs, and finally returning to lysosome after LDs consumption are visualized. In addition, the tracing of dynamic movement and consumption of LDs is realized by the probes with a high signalto-noise ratio. The unique labeling behaviors and distinguished dual emissions of the probes in LDs and lysosome make them promising agents for fluorescence visualization studies of LD-lysosome related bioprocess and metabolism diseases.