Plasminogen activators and their inhibitors in synovial fluids from normal, osteoarthritis, and rheumatoid arthritis knees
Plasminogen activators and their inhibitors in synovial fluids from normal, osteoarthritis, and rheumatoid arthritis knees
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DOI：
10.1136/ard.55.4.230
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发表时间：
1996-04-01
影响因子：
27.4
通讯作者：
Doherty, M
中科院分区：
文献类型：
--
作者：
Belcher, C;Fawthrop, F;Doherty, M
目的建立正常膝关节滑液中纤溶酶原激活剂及其抑制剂的基线浓度，并将其与骨关节炎（OA）和类风湿关节炎（RA）膝关节液进行比较。方法：正常人26例，OA患者71例，RA患者17例，行膝关节抽吸术。根据结节性全身性OA （NGOA）和滑膜液焦磷酸钙晶体的存在对OA患者进行亚分类。对OA和RA患者进行炎症的临床评估（分级0-6）。采用酶联免疫吸附法测定纤溶酶原激活物（PA）、纤溶酶原激活物抑制剂（PAI）和尿激酶型PA受体（uPAR）抗原浓度。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法测定了样品中PAs的种类。结果：RA中所有抗原（uPA、组织型PA （tPA）、uPAR、PAI-1）浓度均显著高于OA；OA组明显高于正常值。其浓度与临床评估的膝关节炎症无直接关系。在正常液体中，没有观察到与年龄的关联。NGOA的抗原浓度（uPA、tPA和uPAR）与其他OA亚类不同，但PA的种类在不同疾病组之间似乎没有变化。优势PA似乎与uPA具有同一性。结论：由于OA中这些抗原的浓度高于正常体液，OA不能作为PA/PAI系统研究的替代正常对照。PA/PAI系统的改变在RA和OA膝关节液体中得到证实，RA的变化更明显。与其他OA液体相比，在NGOA中发现不同浓度的PA抗原进一步支持了该亚群的不同致病机制。
Objectives-To establish baseline concentrations of plasminogen activators and their inhibitors in normal knee synovial fluids, and to compare them with well characterised osteoarthritis (OA) and rheumatoid arthritis (RA) knee fluids.Methods-A total of 26 normal subjects, 71 patients with OA, and 17 patients with RA underwent knee aspiration. Patients with OA were subclassified according to presence of nodal generalised OA (NGOA) and synovial fluid calcium pyrophosphate crystals. Clinical assessment of inflammation (graded 0-6) was undertaken in OA and RA patients. Plasminogen activator (PA), plasminogen activator inhibitor (PAI), and urokinase-type PA receptor (uPAR) antigen concentrations were determined by enzyme linked immunosorbent assay. The species of PAs present were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.Results-Concentrations of all antigens (uPA, tissue-type PA (tPA), uPAR, and PAI-1), were significantly greater in RA than OA; those in OA were significantly greater than normal. The concentrations showed no direct association with clinically assessed inflammation of the knee. In normal fluids, no associations with age were observed. Antigen concentrations (uPA, tPA, and uPAR) in NGOA differed from those in other subclasses of OA, but the species of PA present did not appear to vary between disease groups. The predominant PA appeared to have identity with uPA.Conclusion-Because of the greater concentrations of these antigens in OA compared with normal fluids, OA cannot be used as a surrogate normal control in studies of the PA/PAI system. Alteration of the PA/PAI system was confirmed in RA and OA knee fluids, with greater changes evident in RA. The finding of different concentrations of PA antigens in NGOA compared with other OA fluids further supports a different pathogenic mechanism in this subset.