Anther Culture Response of Barley Genotypes to Cold Pretreatments and Culture Media
Anther Culture Response of Barley Genotypes to Cold Pretreatments and Culture Media
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大麦基因型对冷预处理和培养基的花药培养反应
DOI：
10.1007/s11183-005-0104-8
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发表时间：
2005
影响因子：
1.4
通讯作者：
D. Roupakias
中科院分区：
文献类型：
--
作者：
T. Lazaridou;A. Lithourgidis;S. Kotzamanidis;D. Roupakias
三种大麦花药培养中的胚状体形成和植株再生两个品种(Niki、Karina、Thermi)、一个F1杂种(Niki×Thermi)、两个F2群体(Niki×Thermi、Niki×Karina)和两个F3群体(Niki×Thermi、Niki×Karina)在4℃冷预处理14天和28天后，在两个固体诱导培养基上进行了观察。所使用的培养基(N6和FHG)的组成和能量来源不同(FHG中的麦芽糖与N6中的蔗糖)。诱导基组分和冷预处理对胚状体频率和绿苗再生均有影响。FHG诱导培养基与28天冷预处理的组合对单倍体胚状体的形成和绿苗的产生效果最好。此外，绿色植物的产量也是由基因决定的。简历。Thermi和杂交一代Niki×Thermi的绿苗产生频率最高。花药培养中胚状体形成频率较高或中等的亲本，可有效地从F1或F2世代中产生绿苗，用于育种。
The embryoid formation and plant regeneration in anther cultures of three barley (Hordeum vulgareL.) cultivars (Niki, Karina, Thermi), one F1hybrid (Niki × Thermi), two F2populations (Niki × Thermi, Niki × Karina), and two F3populations (Niki × Thermi, Niki × Karina) were investigated in two solid induction media after cold pretreatment for 14 and 28 days at 4°C . The media used (N6 and FHG) differed in their composition and source of energy (maltose in FHG vs. sucrose in N6). Embryoid frequency and green plant regeneration depended on both the induction medium composition and cold pretreatment. The combination of the FHG induction medium with 28-day-long cold pretreatment was the most efficient in haploid embryoid formation and green plant production. In addition, the green plant production was genotype-dependent. Cv. Thermi and F1hybrid Niki × Thermi exhibited the highest frequency of green plant production. The parent with high or even moderate frequency of embryoid formation in anther culture could lead to the effective production of green plants from the F1hybrid or the F2generation for breeding purposes.