Phasing electron diffraction data by molecular replacement: strategy for structure determination and refinement.
Phasing electron diffraction data by molecular replacement: strategy for structure determination and refinement.
复制标题
通过分子替换定相电子衍射数据：结构确定和细化的策略。
DOI：
10.1007/978-1-62703-176-9_14
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Gonen,Tamir
中科院分区：
文献类型：
--
作者：
Wisedchaisri,Goragot;Gonen,Tamir
电子晶体学可以说是唯一的电子低温显微镜（cryo EM）技术，能够提供原子分辨率数据（优于3 μ m）的膜蛋白嵌入在膜。硬件改进和衍射分析样品制备方面的进展导致了最近一些实现越来越高分辨率的例子。本书的其他章节详细介绍了硬件的改进，并深入研究了显微镜和电子衍射数据收集和处理的样品制备的复杂艺术。在这一章中，我们详细描述了电子衍射研究的分子置换的协议。用于定相电子衍射数据的搜索模型的使用基本上消除了获取图像数据的需要，使得其不受来自漂移和充电效应的像差的影响，漂移和充电效应有效地降低了可达到的分辨率。
Electron crystallography is arguably the only electron cryomicroscopy (cryo EM) technique able to deliver atomic resolution data (better then 3 Å) for membrane proteins embedded in a membrane. The progress in hardware improvements and sample preparation for diffraction analysis resulted in a number of recent examples where increasingly higher resolutions were achieved. Other chapters in this book detail the improvements in hardware and delve into the intricate art of sample preparation for microscopy and electron diffraction data collection and processing. In this chapter, we describe in detail the protocols for molecular replacement for electron diffraction studies. The use of a search model for phasing electron diffraction data essentially eliminates the need of acquiring image data rendering it immune to aberrations from drift and charging effects that effectively lower the attainable resolution.