Critical stresses for cancer cell detachment in microchannels
Critical stresses for cancer cell detachment in microchannels
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DOI：
10.1007/s00249-009-0506-1
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发表时间：
2009-10-01
影响因子：
2
通讯作者：
Verdier, Claude
中科院分区：
文献类型：
--
作者：
Couzon, Cecile;Duperray, Alain;Verdier, Claude
我们目前的实验涉及癌细胞粘附到微通道，受到不断增加的剪切应力（0.1-30帕）。在不同的剪切应力下进行形态学研究。只要剪切应力不太高，细胞表现出与静态条件下观察到的相似的铺展模式。在临界壁剪切应力（约2-5 Pa）下，细胞-基底接触面积减小，直到在较大应力下分离。临界剪切应力被发现是较低的更高的约束（即较小的细胞高度通道高度比）。荧光技术被用来定位在不同剪切条件下的粘着斑（典型的大小为1 μ m（2）），表明细胞增加了面对血流区域的粘着斑数量。为了分析这些数据，我们提出了一个模型来确定临界应力，导致从水动力和粘附细胞阻力之间的竞争。利用该模型，可以确定在每个焦点接触处施加的典型粘附应力，并且与以前的工作一致。
We present experiments involving cancer cells adhering to microchannels, subjected to increasing shear stresses (0.1-30 Pa). Morphological studies were carried out at different shear stresses. Cells exhibit spreading patterns similar to those observed under static conditions, as long as the shear stress is not too high. At critical wall shear stresses (around 2-5 Pa), cell-substrate contact area decreases until detachment at the larger stresses. Critical shear stresses are found to be lower for higher confinements (i.e. smaller cell height to channel height ratio). Fluorescent techniques were used to locate focal adhesions (typically 1 mu m(2) in size) under various shearing conditions, showing that cells increase the number of focal contacts in the region facing the flow. To analyze such data, we propose a model to determine the critical stress, resulting from the competition between hydrodynamic forces and the adhesive cell resistance. With this model, typical adhesive stresses exerted at each focal contact can be determined and are in agreement with previous works.