Interstitial Cajal-like cells in human gallbladder
Interstitial Cajal-like cells in human gallbladder
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DOI：
10.1007/s10735-007-9099-0
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发表时间：
2007-08-01
影响因子：
3.2
通讯作者：
Popescu, Laurentziu M.
中科院分区：
文献类型：
--
作者：
Hinescu, Mihail E.;Ardeleanu, Carmen;Popescu, Laurentziu M.
我们在这里描述了一个人胆囊间质Cajal样细胞类型（ICLC），类似于原型肠间质细胞的Cajal。胆囊ICLC在新鲜制备物（组织冷冻切片）中使用亚甲基蓝显示，固定标本在Epon半薄切片中用甲苯胺蓝或透射电子显微镜（TEM）染色。免疫组化检测CD 117/c-kit、CD 34、vimentin、desmin、nestin、α-平滑肌肌动蛋白、NK-1、S-100、PGP-9.5、tau蛋白、嗜铬粒蛋白A、NSE、GFAP、CD 1a、CD 62-P、CD 68、雌激素受体和孕激素受体等16种抗原进一步证实胆囊ICLC的阳性诊断。分别对CD 117、CD 34和CD 117和巢蛋白进行双重免疫染色。新鲜胆囊ICLC的空间密度为100-110个/mm ~ 2。ICLC主要出现在上皮下和肌层（分别约为7%和5%）。胆囊内胆管癌占胆囊上皮下细胞的5.5%。TEM显示ICLC符合诊断标准。透射电镜观察表明，ICLC的主要超微结构特征，即细胞突起，在相对早期的发育阶段发育成特征性的形状。在人类中，ICLC是否参与胆囊功能（例如起搏、分泌（自分泌、分泌和/或旁分泌）、细胞间信号传导或结石形成）仍有待确定。
We describe here an interstitial Cajal-like cell type (ICLC) in human gallbladder, resembling the archetypal enteric interstitial cells of Cajal. Gallbladder ICLC were demonstrated in fresh preparations (tissue cryosections) using methylene-blue, and fixed specimens in Epon semi-thin sections stained with toluidine blue or transmission electron microscopy (TEM). The positive diagnosis of gallbladder ICLC was further verified by immunohistochemistry: CD117/c-kit, CD34, and another 16 antigens: vimentin, desmin, nestin, alpha-smooth muscle actin, NK-1, S-100, PGP-9.5, tau protein, chromogranin A, NSE, GFAP, CD1a, CD62-P, CD68, estrogen and progesterone receptors. Double immunostaining was performed for CD117, CD34 and CD117 and nestin, respectively. In fresh specimens, the spatial density of gallbladder ICLC was 100-110 cells/mm(2). ICLC mainly appeared beneath the epithelium and in muscularis (about 7%, and similar to 5%, respectively). In toto, ICLC represent in gallbladder similar to 5.5% of subepithelial cells. TEM showed that diagnostic criteria were fulfilled by ICLC. Moreover, TEM indicated that the main ultrastructural distinctive feature for ICLC, the cell processes, develop into the characteristic shape at a relatively early stage of development. It remains to be established if, in humans, ICLC are involved in gallbladder (dis)functions (e.g. pace-making, secretion (auto-, juxta- and/or paracrine), intercellular signaling, or stone formation).