Glomerular crescents predominantly express cadherin-catenin complex in pauci-immune-type crescentic glomerulonephritis
Glomerular crescents predominantly express cadherin-catenin complex in pauci-immune-type crescentic glomerulonephritis
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DOI：
10.1046/j.1365-2559.2003.01660.x
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发表时间：
2003-08-01
期刊：
影响因子：
6.4
通讯作者：
Nagata, M
中科院分区：
文献类型：
--
作者：
Usui, J;Kanemoto, K;Nagata, M
目的：研究钙粘蛋白复合物在人月牙状肾小球肾炎中的表达，以阐明细胞间粘附连接分子在月牙状肾炎形成中的作用。方法和结果：免疫染色显示正常人肾小球的鲍曼上皮细胞中有钙粘蛋白复合物，而足细胞中没有。本文对8例髓过氧化物酶抗中性粒细胞胞浆自身抗体（MPO-ANCA）相关（pauci-免疫型）月牙型肾小球肾炎患者进行免疫荧光显微镜检查，检测其抗pan - cadherin、p120 catenin和β -catenin抗体。这些标本提供了6个细胞月牙，12个纤维细胞月牙和4个纤维化月牙。免疫荧光根据四个等级系统[(-)-(++)]，通过在新月的整个区域内定位场的比率进行半定量估计。所有由钙粘蛋白复合物组成的测试分子都在月牙细胞角蛋白阳性的上皮成分中大量观察到，每个分子都具有相同的定位面积。钙粘蛋白复合物的表达与细胞角蛋白的表达密切相关，并且随着新月从细胞向纤维化的发展，两者的表达都减少了。结论：钙粘蛋白-连环蛋白复合物是人肾小球鲍曼上皮细胞的特异性标志物。包囊免疫型月牙状肾小球肾炎的月牙状细胞具有粘附连接分子，提示主要的壁上皮细胞表型。
Aims: To investigate the expression of the cadherin complex in human crescentic glomerulonephritis to elucidate the role of intercellular adherens junction molecules in crescent formation.Methods and results: Immunostaining revealed cadherin complexes localized in Bowman's epithelial cells, but not in podocytes, of normal human glomeruli. Eight adult cases with myeloperoxidase anti-neutrophil cytoplasmic autoantibodies (MPO-ANCA)-related (pauci-immune type) crescentic glomerulonephritis were examined on immunofluorescence microscopy with anti-pan cadherin, p120 catenin, and beta-catenin antibodies. The specimens provided six cellular crescents, 12 fibrocellular crescents, and four fibrotic crescents. Immunofluorescence was semiquantitatively estimated by the rate of the field of localization within the whole area of the crescent, according to the four-grade system [(-) - (++)]. All the tested molecules consisting of the cadherin complex were abundantly observed in cytokeratin-positive epithelial components in crescents, each with an equivalent area of localization. The expression of the cadherin complex was closely associated with that of cytokeratin and both diminished as the crescents developed from cellular to fibrotic.Conclusions: The cadherin-catenin complex is a specific marker of Bowman's epithelial cells in human glomeruli. The cellular crescents in pauci-immune-type crescentic glomerulonephritis possess adherens junction molecules, indicating a principle parietal epithelial cell phenotype.