NULL MUTATION OF DLX-2 RESULTS IN ABNORMAL MORPHOGENESIS OF PROXIMAL FIRST AND 2ND BRANCHIAL ARCH DERIVATIVES AND ABNORMAL DIFFERENTIATION IN THE FOREBRAIN
NULL MUTATION OF DLX-2 RESULTS IN ABNORMAL MORPHOGENESIS OF PROXIMAL FIRST AND 2ND BRANCHIAL ARCH DERIVATIVES AND ABNORMAL DIFFERENTIATION IN THE FOREBRAIN
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DOI：
10.1101/gad.9.20.2523
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发表时间：
1995-10-15
影响因子：
10.5
通讯作者：
RUBENSTEIN, JLR
中科院分区：
文献类型：
--
作者：
QIU, MS;BULFONE, A;RUBENSTEIN, JLR
对脊椎动物头部发育和进化的遗传学分析还处于原始阶段。许多同源盒基因，包括Distal-less家族，是头部发育的潜在调节因子。为了确定DIx-2的功能，我们使用基因靶向在小鼠中产生了无效突变。在纯合子突变体中，前脑内的分化是异常的，并且颅神经嵴细胞的子集的命运被重新指定。后者导致来自第一和第二鳃弓近端部分的骨骼元素的异常形态发生。我们假设，第一个足弓的受影响头骨已经经历了一个类似于爬行动物的结构转变。这些结果表明Dlx-2控制鳃弓和前脑的发育，并表明其在颅面进化中的作用。
Genetic analysis of the development and evolution of the vertebrate head is at a primitive stage. Many homeo box genes, including the Distal-less family, are potential regulators of head development. To determine the function of DIx-2, we generated a null mutation in mice using gene targeting. In homozygous mutants, differentiation within the forebrain is abnormal and the fate of a subset of cranial neural crest cells is respecified. The latter causes abnormal morphogenesis of the skeletal elements derived from the proximal parts of the first and second branchial arches. We hypothesize that the affected skull bones from the first arch have undergone a transformation into structures similar to those found in reptiles. These results show that Dlx-2 controls development of the branchial arches and the forebrain and suggests its role in craniofacial evolution.