Characterization of lipid extracts from brain tissue and tumors using Raman spectroscopy and mass spectrometry
Characterization of lipid extracts from brain tissue and tumors using Raman spectroscopy and mass spectrometry
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DOI：
10.1007/s00216-008-2592-9
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发表时间：
2009-03-01
影响因子：
4.3
通讯作者：
Krafft, Christoph
中科院分区：
文献类型：
--
作者：
Koehler, Milena;Machill, Susanne;Krafft, Christoph
脑组织的特点是脂质含量高。在最常见的原发性脑肿瘤胶质瘤中，脂质的量减少，其组成发生变化。本论文的范围是使用Folch的改良方案从猪脑和人脑组织以及5个人脑胶质瘤中定量提取脂质。通过具有785 nm激发的拉曼光谱法和具有电子碰撞电离的质谱法研究脂质提取物。猪脑组织和人脑组织具有相似的水和脂质含量，并显示相似的拉曼和质谱。相反，神经胶质瘤的特征在于水含量增加和脂质含量降低。胆碱组和胆固醇的拉曼谱带显示胶质瘤脂质提取物中磷脂酰胆碱与胆固醇的比例升高。由于其更高的灵敏度，质谱检测到相对于神经胶质瘤的脂质提取物中的胆固醇，胆固醇酯的水平增加。为了比较，在脂质提取之前从胶质瘤标本制备薄组织切片;记录红外光谱图像并通过监督分类模型进行分析。这项研究展示了如何改善脑肿瘤的分析，并补充使用多模态方法的脑病理诊断。
Brain tissue is characterized by high lipid content. The amount of lipids decreases, and its composition changes in the most frequent primary brain tumor, the glioma. Scope of the current paper was to extract quantitatively lipids from porcine and human brain tissue as well as from five human gliomas using a modified protocol according to Folch. The lipid extracts were studied by Raman spectroscopy with 785 nm excitation and by mass spectrometry with electron impact ionization. Porcine and human brain tissues have similar water and lipid content and show similar Raman and mass spectra. In contrast, gliomas are characterized by increased water content and decreased lipid content. Elevated phosphatidylcholine to cholesterol ratios in lipid extracts of gliomas were indicated by Raman bands of the choline group and cholesterol. Due to its higher sensitivity, mass spectrometry detected increased levels of cholesterol ester relative to cholesterol in lipid extracts of gliomas. For comparison, thin tissue sections were prepared from the glioma specimens before lipid extraction; infrared spectroscopic images were recorded and analyzed by a supervised classification model. This study demonstrates how to improve the analysis of brain tumors and to complement the diagnosis of brain pathologies using a multimodal approach.