Increased serum soluble CD14, ICAM-1 and E-selectin correlate with disease activity and prognosis in systemic lupus erythematosus
Increased serum soluble CD14, ICAM-1 and E-selectin correlate with disease activity and prognosis in systemic lupus erythematosus
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DOI：
10.1191/096120300678828749
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发表时间：
2000-01-01
期刊：
影响因子：
2.6
通讯作者：
Dörner, T
中科院分区：
文献类型：
--
作者：
Egerer, K;Feist, E;Dörner, T
为了改善对免疫和疾病活动的监测，我们测定了系统性红斑狼疮（SLE）和原发性干燥综合征（pSS）患者中单核细胞/巨噬细胞系统（sCD14）和血管内皮（se -选择素，sICAM-1）活性的可溶性标志物，并将其与感染或败血症患者进行比较。采用ELISA系统检测SLE、pSS、脓毒症患者、不同传染病患者和健康对照患者血清中sCD14、sICAM-1和se -选择素（分别为可溶性CD14、ICAM-1和e -选择素）的浓度。与局部感染患者相比，SLE和败血症患者检测到se -选择素和sICAM-1水平升高（SLE和败血症分别与感染相比P < 0.001； Kruskal-Wallis检验）。SLE患者血清中sCD14水平持续升高，而脓毒症或感染患者在接受有效治疗后，sCD14水平迅速下降。这三个参数的持续升高与败血症患者和SLE患者的致命结局相关。sCD14、sICAM-1和se -选择素的联合升高与活动性SLE患者的预后相关，表明单核/巨噬细胞系统和内皮的显著免疫激活与仅在败血症患者中发现的激活相当。
To improve monitoring of immunological and disease activity, we determined soluble markers of activity of the monocyte/macrophage system (sCD14) and the vascular endothelium (sE-selectin, sICAM-1) in patients with systemic lupus erythematosus (SLE) and primary Sjogren's syndrome (pSS) in comparison to patients with infections or sepsis.Concentrations of sCD14, sICAM-1 and sE-selectin (soluble CD14, ICAM-1 and E-selectin, respectively) were measured in serum samples from patients with SLE and pSS, patients with sepsis, different infectious diseases and healthy controls using ELISA systems.Elevated levels of sE-selectin and sICAM-1 were detected in patients with SLE as well as sepsis, in contrast to patients with a localized infection (SLE and sepsis, respectively, versus infection P < 0.001; Kruskal-Wallis test). Levels of sCD14 were persistently elevated in sera from patients with SLE, whereas these values decreased rapidly after effective therapy in patients with sepsis or infection. A continuous elevation of all of these three parameters was associated with a fatal outcome in patients with sepsis as well as in patients with SLE.Combined elevation of sCD14, sICAM-1 and sE-selectin correlates with the prognosis in patients with active SLE and indicates a remarkable immune activation involving the monocyte/macrophage system and the endothelium comparable to an activation found only in patients with sepsis.