Transcriptional regulation of an Arabidopsis gene encoding a CCT domain-containing protein during endoplasmic reticulum stress
Transcriptional regulation of an Arabidopsis gene encoding a CCT domain-containing protein during endoplasmic reticulum stress
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DOI：
10.5511/plantbiotechnology.25.397
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Koizumi, Nozomu
中科院分区：
文献类型：
--
作者：
Iwata, Yuji;Yamada, Takeo;Koizumi, Nozomu
在真核细胞中，内质网 (ER) 中未折叠蛋白的积累会触发 ER 驻留分子伴侣和折叠酶的转录诱导，以维持细胞稳态，称为 ER 应激反应或未折叠蛋白反应 (UPR)。在这项研究中，我们重点关注拟南芥的假定转录调节因子，其转录物响应内质网应激诱导剂衣霉素而积累。该基因被命名为 TCR1（衣霉素诱导的 CONSTANS 样相关 1），编码 195 个氨基酸的预测蛋白，具有植物特异性 CCT（CONSTANS、CONSTANS 样、TOC1）结构域，被认为在转录调节中发挥作用。在使用拟南芥原生质体的双荧光素酶测定中，我们发现衣霉素对 TCR1 基因的诱导不依赖于与其启动子上的顺式元件 ER 应激反应元件相似的核苷酸序列。我们的结果进一步表明这种诱导可能是由转录因子 AtbZIP60 介导的。这项研究是了解 TCR1 在拟南芥 ER 应激反应中作用的第一步。
In eukaryotic cells, accumulation of unfolded proteins in the endoplasmic reticulum ( ER) triggers the transcriptional induction of ER-resident molecular chaperones and folding enzymes to maintain cellular homeostasis, termed the ER stress response or the unfolded protein response (UPR). In this study we focused on a putative transcriptional regulator of Arabidopsis thaliana whose transcripts accumulate in response to the ER stress inducer tunicamycin. This gene, designated as TCR1 (tunicamycin-induced CONSTANS-like-related 1), encodes a predicted protein of 195 amino acids with a plant-specific CCT ( CONSTANS, CONSTANS-like, TOC1) domain considered to function in transcriptional regulation. In a dual-luciferase assay using Arabidopsis protoplasts, we showed that induction of the TCR1 gene by tunicamycin is independent of a nucleotide sequence similar to the cis-element ER stress response element on its promoter. Our result further indicates that this induction could be mediated by the transcription factor AtbZIP60. This study is a first step to understand the role of TCR1 in the Arabidopsis ER stress response.