WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes
WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes
复制标题
WHAMM 是小鼠卵母细胞减数分裂纺锤体迁移和不对称胞质分裂所必需的
DOI：
10.1007/s00418-012-1051-z
复制
发表时间：
2013-04-01
影响因子：
2.3
通讯作者：
Wang, Hai-Long
中科院分区：
文献类型：
--
作者：
Huang, Xin;Ding, Lu;Wang, Hai-Long
与肌动蛋白、膜和微管相关的WASP同源物（WHAMM）是一种新发现的促核因子，它连接肌动蛋白和微管细胞骨架，并调节从内质网到高尔基体的运输。然而，对WHAMM的认识仅限于间期体细胞。在本研究中，我们检测了它在小鼠卵母细胞减数分裂过程中的定位和功能。免疫染色显示，生发囊（GV）期未见WHAMM信号；减数分裂恢复后，WHAMM在前中期I （Pro MI）、中期I （MI）、末期I （TI）和中期II （MII）阶段与纺锤体相关。诺可达唑和紫杉醇处理表明，WHAMM定位于心肌纺锤体周围。通过向卵母细胞细胞质中微量注射特异性短干扰RNA （si）来消耗WHAMM，导致纺锤体迁移失败，不对称细胞质分裂中断，减数分裂成熟过程中第一极体挤压率降低。此外，在WHAMM耗尽后，肌动蛋白帽的形成也被破坏，证实纺锤体迁移失败。综上所述，我们的数据表明，在小鼠卵母细胞成熟过程中，WHAMM是外周纺锤体迁移和不对称细胞质分裂所必需的。
WASP homolog associated with actin, membranes and microtubules (WHAMM) is a newly discovered nucleation-promoting factor that links actin and microtubule cytoskeleton and regulates transport from the endoplasmic reticulum to the Golgi apparatus. However, knowledge of WHAMM is limited to interphase somatic cells. In this study, we examined its localization and function in mouse oocytes during meiosis. Immunostaining showed that in the germinal vesicle (GV) stage, there was no WHAMM signal; after meiosis resumption, WHAMM was associated with the spindle at prometaphase I (Pro MI), metaphase I (MI), telophase I (TI) and metaphase II (MII) stages. Nocodazole and taxol treatments showed that WHAMM was localized around the MI spindle. Depletion of WHAMM by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in failure of spindle migration, disruption of asymmetric cytokinesis and a decrease in the first polar body extrusion rate during meiotic maturation. Moreover, actin cap formation was also disrupted after WHAMM depletion, confirming the failure of spindle migration. Taken together, our data suggest that WHAMM is required for peripheral spindle migration and asymmetric cytokinesis during mouse oocyte maturation.