Rejoining and misrejoining of radiation-induced chromatin breaks. I. experiments with human lymphocytes.
Rejoining and misrejoining of radiation-induced chromatin breaks. I. experiments with human lymphocytes.
复制标题
辐射引起的染色质断裂的重新连接和错误重新连接。
作者：
M. Durante;K. George;H. Wu;T. Yang
Fluorescence in situ hybridization with a composite probe for human chromosome 4 and a probe that stained all centromeres was used to study gamma-ray induced breakage, rejoining and misrejoining in prematurely condensed chromosomes in human lymphocytes. Dose-response curves for the induction of all types of aberrations in prematurely condensed human chromosomes 4 were determined immediately after irradiation and after 8 h postirradiation incubation. In addition, aberrations were measured after various incubation times from 0 to 18 h after a dose of 7 Gy. Unrejoined chromosome breaks were the most frequent type of aberration observed immediately after irradiation. Approximately 15% of total aberrations observed were chromosome exchanges. After 8 h postirradiation incubation, the frequency of breaks in prematurely condensed chromosomes declined to about 20% of the initial value, and chromosomal exchanges became the most frequent aberration. Results of metaphase analysis were similar to those for prematurely condensed chromosomes after 8 h incubation with the exception that a significantly lower frequency of fragments was observed. Symmetrical and asymmetrical interchanges were found at similar frequencies at all doses. No complex exchanges were observed in lymphocyte chromosomes immediately after exposure. They accounted for about 1% of total exchanges in metaphase chromosomes at doses <3 Gy and about 14% at 7 Gy. Incomplete exchanges amounted to approximately 15% of total exchanges at all doses. The kinetics of break rejoining was exponential, and the frequency of exchanges increased with kinetics similar to that observed for the rejoining of the breaks. This increase in the total exchanges as a function of the time between irradiation and fusion was due to a rapid increase in reciprocal interchanges, and a slower increase in complex exchanges; the frequency of incomplete exchanges increased initially, then decreased with prolonged incubation to the level observed in metaphase. It is concluded that the formation of each kind of chromosome aberrations follows different kinetics.
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DOI：
10.1073/pnas.83.9.2934
发表时间：
1986-05-01
影响因子：
11.1
作者：
PINKEL, D;STRAUME, T;GRAY, JW
通讯作者：
GRAY, JW
影响因子：
3.4
作者：
PANDITA, TK;HITTELMAN, WN
通讯作者：
HITTELMAN, WN
通过荧光原位杂交可视化受辐射的人成纤维细胞中的非互易染色体交换。
影响因子：
3.4
作者：
Brown,JM;Kovacs,MS
通讯作者：
Kovacs,MS
影响因子：
56.9
作者：
CORNFORTH, MN;BEDFORD, JS
通讯作者：
BEDFORD, JS
通过荧光原位杂交检测到染色体大小对伽马辐射引起的畸变水平的影响。
DOI：
10.1159/000133807
发表时间：
1994
期刊：
Cytogenetics and cell genetics
影响因子：
--
作者：
Pandita,TK;Gregoire,V;Dhingra,K;Hittelman,WN
通讯作者：
Hittelman,WN