Associations between hepatic metabolism of propionate and palmitate in liver slices from transition dairy cows.
Associations between hepatic metabolism of propionate and palmitate in liver slices from transition dairy cows.
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围产期奶牛肝切片中丙酸和棕榈酸的肝脏代谢之间的关联。
DOI：
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发表时间：
2015
影响因子：
3.5
通讯作者：
T. Overton
中科院分区：
文献类型：
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作者：
M. McCarthy;M. Piepenbrink;T. Overton
使用经产荷斯坦奶牛（n = 95）评估围产期肝脏丙酸和棕榈酸代谢以及肝脏组成随时间的变化，以及这些变量与围产期非酯化脂肪酸和β-羟基丁酸累积增加的关系。之前 3 次实验的数据用于实现研究目标，总共涉及 95 头经产荷斯坦奶牛。使用与分娩相关的第 -21、1 和 21 天的活检肝切片来测定 [1-(14)C] 棕榈酸酯向 CO2 和酯化产物 (EP) 的转化以及 [1-(14)C] 丙酸酯向 CO2 和葡萄糖的转化。肝糖原含量在第 -21 天最高，第 1 天和第 21 天分别为产前值的 26.9% 和 36.5%。肝脏甘油三酯含量在第 -21 天最低，第 1 天和第 21 天分别为产前值的 271% 和 446%。我们检测到 d -21 和 d 1 之间肝脏将 [1-(14)C] 棕榈酸酯氧化为 CO2 的能力没有差异；然而，在第 21 天，氧化为产前值的 84%。第 1 天和第 21 天，肝脏将 [1-(14)C] 棕榈酸酯转化为 EP 的能力分别是产前值的 148% 和 139%。第1天和第21天，肝脏将[1-(14)C]丙酸转化为CO2的能力分别为产前值的127%和83%，第1天和第21天，肝脏将[1-(14)C]丙酸转化为葡萄糖的能力分别为产前值的126%和85%。相关关系表明，总体而言，产前肝脏甘油三酯含量升高的奶牛在整个围产期甘油三酯升高，同时[1-(14)C]棕榈酸酯氧化和转化为EP的增加以及[1-(14)C]丙酸酯转化为葡萄糖的倾向降低。 [1-(14)C]丙酸氧化增加的奶牛在整个过渡期[1-(14)C]丙酸向葡萄糖的转化率增加。总体而言，导致围产期脂肪酸代谢受损的情况似乎与产后肝脏丙酸代谢受损有关。
Multiparous Holstein cows (n=95) were used to evaluate changes in hepatic propionate and palmitate metabolism and liver composition over time during the transition period, along with the relationships of these variables with cumulative increases in nonesterified fatty acids and β-hydroxybutyrate during the periparturient period. Data from 3 previous experiments were used to address the study objectives, accounting for a total of 95 multiparous Holstein cows. Liver slices from biopsies on d -21, 1, and 21 relative to parturition were used to determine conversion of [1-(14)C]palmitate to CO2 and esterified products (EP) and the conversion of [1-(14)C]propionate to CO2 and glucose. Hepatic glycogen content was highest on d -21 and was 26.9 and 36.5% of prepartum values on d 1 and 21, respectively. Liver triglyceride content was lowest at d -21 and was 271 and 446% of prepartum values on d 1 and 21, respectively. We detected no difference in the capacity for the liver to oxidize [1-(14)C]palmitate to CO2 between d -21 and d 1; however, on d 21, oxidation was 84% of prepartum values. The capacity of the liver to convert [1-(14)C]palmitate to EP was 148 and 139% of prepartum values on d 1 and 21, respectively. The capacity of liver to convert [1-(14)C]propionate to CO2 was 127 and 83% of prepartum values on d 1 and 21, and the capacity of liver to convert [1-(14)C]propionate to glucose was 126 and 85% of prepartum values on d 1 and 21, respectively. Correlation relationships suggest that overall, cows with elevated prepartum liver triglyceride content had elevated triglycerides throughout the transition period along with increased [1-(14)C]palmitate oxidation and conversion to EP and a decreased propensity to convert [1-(14)C]propionate to glucose. Cows with increased [1-(14)C]propionate oxidation had increased conversion of [1-(14)C]propionate to glucose throughout the transition period. Overall, conditions that lead to impairments in fatty acid metabolism during the transition period appear to be associated with impaired postpartum hepatic propionate metabolism.