Studying Culicoides vectors of BTV in the post-genomic era: resources, bottlenecks to progress and future directions.
Studying Culicoides vectors of BTV in the post-genomic era: resources, bottlenecks to progress and future directions.
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DOI：
10.1016/j.virusres.2013.12.009
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发表时间：
2014-03
期刊：
影响因子：
5
通讯作者：
Carpenter S
中科院分区：
文献类型：
--
作者：
Nayduch D;Cohnstaedt LW;Saski C;Lawson D;Kersey P;Fife M;Carpenter S
索氏库蚊是蓝舌病病毒载体中唯一定植的物种。这一物种的完整注释基因组的开发正在进行中。转录分析正被用于研究基因组的功能元件，特别是涉及吞血、繁殖、发育和载体能力的基因。库蚊叮咬蚊(Diptera：Ceratopogonidae)是一个主要的媒介群，负责广泛的全球重要虫媒病毒的生物传播，包括蓝舌病毒(BTV)。在这篇综述中，我们综述了目前研究该属的生物学资源，重点介绍了来自美国BTV的主要媒介--索氏华支睾吸虫的现存菌落和细胞系的历史。然后我们讨论了在载体生物过程的基因组和转录组分析的快速发展领域，并介绍了新成立的库蚊基因组和转录组学联盟。详细介绍了这些领域的初步结果，最后从昆虫学的角度讨论了未来可能研究的领域，描述了我们对库蚊生物学的理解限制，这些限制可能会阻碍这些领域的进展。
Culicoides sonorensis is the only colonized species of bluetongue virus vector. The development of a fully annotated genome for this species is in progress. Transcriptomic analyses are being employed to investigate functional elements of the genome, particularly genes involved in hematophagy, reproduction, development and vector competence. Culicoides biting midges (Diptera: Ceratopogonidae) are a major vector group responsible for the biological transmission of a wide variety of globally significant arboviruses, including bluetongue virus (BTV). In this review we examine current biological resources for the study of this genus, with an emphasis on detailing the history of extant colonies and cell lines derived from C. sonorensis, the major vector of BTV in the USA. We then discuss the rapidly developing area of genomic and transcriptomic analyses of biological processes in vectors and introduce the newly formed Culicoides Genomics and Transcriptomics Alliance. Preliminary results from these fields are detailed and finally likely areas of future research are discussed from an entomological perspective describing limitations in our understanding of Culicoides biology that may impede progress in these areas.