A major nucleolar protein B23 as a marker of proliferation activity of human peripheral lymphocytes
A major nucleolar protein B23 as a marker of proliferation activity of human peripheral lymphocytes
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DOI：
10.1016/s0165-2478(02)00085-8
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发表时间：
2002-08-01
影响因子：
4.4
通讯作者：
Malashenko, OS
中科院分区：
文献类型：
--
作者：
Dergunova, NN;Bulycheva, TI;Malashenko, OS
用一种新的抗核仁磷蛋白B23的单克隆抗体（3C 9），用间接免疫荧光和Western印迹法研究了植物血凝素（PHA）刺激的人外周血淋巴细胞1323个定性和定量变化。结果表明，淋巴细胞增殖伴随着核仁的逐渐增加，其蛋白B23尖端的积累到16小时时为2倍，到72小时时为40-50倍。与未受刺激的细胞相比。通过用抗Ki-67抗体平行免疫标记。结果表明，1323的量和定位的早期变化发生在Ki-67蛋白（一种已知的增殖细胞标志物）出现之前。我们的研究结果表明，针对B23的抗体可用于识别人外周淋巴细胞在其活化增殖的早期阶段，在S期之前。(C)2003 Elsevier Science B. V.保留所有权利。
A novel monoclonal antibody (Mab) (called 3C9) against a major nucleolar phosphoprotein B23 was used to study 1323 qualitative and quantitative alterations in phytohemagglutinin (PHA) -stimulated human peripheral blood lymphocytes in indirect immunofluorescence and Western blots. It was shown that lymphocyte proliferation was accompanied by gradual augmentation of nucleoli and their accumulation of the protein B23 tip to 2-fold by 16 h and 40-50 fold by 72 h. as compared with the non-stimulated cells. By parallel immunolabeling with the anti-Ki-67 antibody. it was shown that the early changes of 1323 amount and localization occurred before an appearance of Ki-67 protein, a ell-known marker of proliferating cells. Our results evidence that antibodies against B23 might be applied for recognition of human peripheral lymphocytes at early stages of their activation for proliferation, preceding the S-phase. (C) 2003 Elsevier Science B.V. All rights reserved.