NIST mixed stain study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity
NIST mixed stain study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity
复制标题
DOI：
10.1021/ac026410i
复制
发表时间：
2003-05-15
影响因子：
7.4
通讯作者：
Butler, JM
中科院分区：
文献类型：
--
作者：
Kline, MC;Duewer, DL;Butler, JM
混合染色研究 3 (MSS3) 实验室间挑战练习使用一组设计浓度和成分的七种 DNA 提取物评估了 STR 多重 DNA 分型系统 2001 年的性能。这份初步报告重点关注 DNA ([DNA]) 浓度测量与观察到的 STR 多重信号强度之间的联系。 [DNA] 测量精度与 STR 多重分析的效率之间存在因果关系。所报告的 [DNA] 测量技术之间不存在内在的测量性能差异。然而，使用相同标称测量系统的参与者之间的扩增、分离和检测效率存在很大差异。
The Mixed Stain Study 3 (MSS3) interlaboratory challenge exercise evaluated the 2001 performance of STR multiplex DNA typing systems using a set of seven DNA extracts of designed concentration and composition. This initial report focuses on the linkages connecting the measurement of the concentration of DNA ([DNA]) to the observed STR multiplex signal intensities. There is a causal relationship between [DNA] measurement accuracy and the efficiency of STR multiplex analysis. There are no intrinsic measurement performance differences among the [DNA] measurement technologies reported. However, there are large differences in the efficiencies of amplification, separation, and detection among participants using the same nominal measurement systems.