Insecticidal bacteria isolated from predatory larvae of the antlion species Myrmeleon bore (Neuroptera: Myrmeleontidae)
Insecticidal bacteria isolated from predatory larvae of the antlion species Myrmeleon bore (Neuroptera: Myrmeleontidae)
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DOI：
10.1016/j.jip.2007.02.007
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发表时间：
2007-09-01
影响因子：
3.4
通讯作者：
Matsuda, Kazuhiko
中科院分区：
文献类型：
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作者：
Nishiwaki, Hisashi;Ito, Katsuhiko;Matsuda, Kazuhiko
从蚁狮 Myrmeleon 的嗉囊（消化器官）中分离出多种细菌，并通过注射测试其对毛毛虫的杀虫活性。根据 16S rRNA 基因序列的同源性和生化特性，从蚁狮作物中分离出的 68 个菌株被分为 24 个物种。当以每只昆虫 5 x 105 个细胞的剂量注射时，分离的蜡样芽孢杆菌、球形芽孢杆菌、摩根氏菌、粘质沙雷氏菌和克雷伯菌属可杀死 80% 或更多的地老虎。此外，被这些分离物杀死的地老虎与被蚁狮攻击的毛毛虫的观察结果相似。一项独立于培养物的分析表明，分离出的细菌物种可能经常存在于蚁狮作物中。这些结果表明，杀虫微生物与蚁狮相关，并可能促进猎物的死亡。 (c) 2007 Elsevier Inc. 保留所有权利。
Various bacterial species were isolated from the crop (digestive organ) of the antlion species Myrmeleon bore and tested for their insecticidal activity against caterpillars by injection. Sixty-eight isolates from the antlion crop were grouped into twenty-four species based on homologies of 16S rRNA gene sequences and biochemical properties. Isolated Bacillus cereus, Bacillus sphaericus, Morganella morganii, Serratia marcescens and a Klebsiella species killed 80% or more cutworms when injected at a dose of 5 x 105 cells per insect. In addition, cutworms killed by these isolates resembled observations made of caterpillars attacked by antlions. A culture-independent analysis showed that the isolated bacterial species are likely to be frequently present in the antlion crop. These results suggest that insecticidal microorganisms associate with antlions, and may promote the death of prey. (c) 2007 Elsevier Inc. All rights reserved.