Restriction Endonuclease Cleavage Maps of the Ampicillin Transposons Tn2601 and Tn2602
Restriction Endonuclease Cleavage Maps of the Ampicillin Transposons Tn2601 and Tn2602
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氨苄青霉素转座子 Tn2601 和 Tn2602 的限制性内切酶切割图谱
DOI：
10.1111/j.1348-0421.1980.tb02919.x
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发表时间：
1980
影响因子：
2.6
通讯作者：
S. Yamagishi
中科院分区：
文献类型：
--
作者：
Tomoko Yamamoto;S. Yamagata;Yohko Hashimoto;S. Yamagishi
本文报道了氨苄青霉素转座子Tn2601和Tn2602对限制性内切酶BamHI、Pvu Ⅱ、Avlidin、HincII和Hae Ⅱ的酶切图谱。这两个转座子在大小、核酸内切酶切割位点和在两端具有短的反向重复序列方面与众所周知的氨苄青霉素转座子Tn3非常相似。在BamHI位点周围的区域中观察到这三个转座子之间的切割模式的轻微差异，该区域被认为是用于转座的阻遏基因的一部分。
This paper reports the cleavage maps of ampicillin transposons Tn2601 and Tn2602, for restriction endonucleases BamHI, PvuII, AvaI, HincII, and HaeII. Both of the transposons are very similar to the well‐known ampicillin transposon Tn3 in size, endonuclease cleavage sites, and possession of a short inverted repeat sequence at both ends. A slight difference in the cleavage pattern among these three transposons was observed in the region around the BamHI site which was assumed to be a part of the repressor gene for transposition.