Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone
Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone
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DOI：
10.1002/cm.21032
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发表时间：
2012-06
期刊：
影响因子：
2.9
通讯作者：
Akihiro C E Shibata;T. Fujiwara;Limin H Chen;Kenichi G. N. Suzuki;Yoshiro Ishikawa;Yuri L. Nemoto;Y. Miwa;Z. Kalay;R. Chadda;K. Naruse;A. Kusumi
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文献类型：
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作者：
Akihiro C E Shibata;T. Fujiwara;Limin H Chen;Kenichi G. N. Suzuki;Yoshiro Ishikawa;Yuri L. Nemoto;Y. Miwa;Z. Kalay;R. Chadda;K. Naruse;A. Kusumi
粘着斑（FA）是一种构建在质膜内/上的基于整合素的结构，以机械方式将细胞外基质与肌动蛋白应力纤维的末端连接起来，为细胞在组织中迁移提供关键支架。 FA被认为是微米级的、各种蛋白质的大量组装，尽管它的形成和分解在几分钟到几十秒内迅速发生。 FA 快速调节的机制一直是细胞生物学中的一个重大谜团。在这里，使用快速单荧光分子成像，我们发现转铁蛋白受体和 Thy1（非 FA 膜蛋白）很容易进入 FA 区，在那里快速扩散，并退出到本体质膜中。整合素β3也很容易进入FA区，并在FA区反复进行暂时固定和扩散，而大约三分之一的整合素β3被固定在那里。这些结果与 FA 的群岛结构一致，FA 由许多整合素岛组成：膜分子相当自由地进入岛间通道，整合素岛中的整合素可以与体膜中的整合素快速交换。这种群岛结构将允许 FA 快速形成和分解，并且可能适用于质膜中的其他大蛋白质结构域。 © 2012 Wiley 期刊公司
The focal adhesion (FA) is an integrin‐based structure built in/on the plasma membrane, mechanically linking the extracellular matrix with the termini of actin stress fibers, providing key scaffolds for the cells to migrate in tissues. The FA was considered as a micron‐scale, massive assembly of various proteins, although its formation and decomposition occur quickly in several to several 10 s of minutes. The mechanism of rapid FA regulation has been a major mystery in cell biology. Here, using fast single fluorescent‐molecule imaging, we found that transferrin receptor and Thy1, non‐FA membrane proteins, readily enter the FA zone, diffuse rapidly there, and exit into the bulk plasma membrane. Integrin β3 also readily enters the FA zone, and repeatedly undergoes temporary immobilization and diffusion in the FA zone, whereas approximately one‐third of integrin β3 is immobilized there. These results are consistent with the archipelago architecture of the FA, which consists of many integrin islands: the membrane molecules enter the inter‐island channels rather freely, and the integrins in the integrin islands can be rapidly exchanged with those in the bulk membrane. Such an archipelago architecture would allow rapid FA formation and disintegration, and might be applicable to other large protein domains in the plasma membrane. © 2012 Wiley Periodicals, Inc