Low-Saturation-Intensity, High-Photostability, and High-Resolution STED Nanoscopy Assisted by CsPbBr3 Quantum Dots
Low-Saturation-Intensity, High-Photostability, and High-Resolution STED Nanoscopy Assisted by CsPbBr3 Quantum Dots
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CsPbBr3 量子点辅助的低饱和强度、高光稳定性和高分辨率 STED 纳米显微镜
DOI：
10.1002/adma.201800167
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发表时间：
2018
影响因子：
29.4
通讯作者：
Qu Junle
中科院分区：
文献类型：
--
作者：
Ye Shuai;Yan Wei;Zhao Mengjie;Peng Xiao;Song Jun;Qu Junle
受激发射耗尽（STED）纳米显微镜是用于微结构成像的最有前途的超分辨率成像技术之一。采用商用CdSe@ZnS量子点作为STED探针，获得了近50 nm的横向分辨率。与其他量子点相比，钙钛矿CsPbBr 3纳米颗粒（NPs）具有更高的光致发光量子产率和更大的吸收截面，使其成为STED纳米显微镜更有效的探针。在这项研究中，CsPbBr 3 NPs被用作STED纳米成像的探针。CsPbBr 3样品的荧光强度在用39.8mW的耗尽激光照射200分钟后几乎没有减弱，表明CsPbBr 3纳米颗粒具有优异的耐光漂白性。CsPbBr 3 NP的饱和强度极低，估计仅为0.4 mW（0.126 MW cm-2）。最后，在基于CsPbBr 3的STED纳米显微成像中，在27.5 mW STED激光照射下，单个纳米颗粒获得了20.6 nm的横向分辨率，与共聚焦显微镜相比，这是十倍的改进。由于其在较低耗尽功率下的高荧光稳定性和高分辨率，CsPbBr 3辅助STED纳米显微镜在研究需要超分辨率和长期成像的微观结构方面具有巨大的潜力。
Stimulated emission depletion (STED) nanoscopy is one of the most promising super‐resolution imaging techniques for microstructure imaging. Commercial CdSe@ZnS quantum dots are used as STED probes and ≈50 nm lateral resolution is obtained. Compared with other quantum dots, perovskite CsPbBr3nanoparticles (NPs) possess higher photoluminescence quantum yield and larger absorption cross‐section, making them a more effective probe for STED nanoscopy. In this study, CsPbBr3NPs are used as probes for STED nanoscopy imaging. The fluorescence intensity of the CsPbBr3sample is hardly weakened at all after 200 min irradiation with a 39.8 mW depletion laser, indicating excellent photobleaching resistance of the CsPbBr3NPs. The saturation intensity of the CsPbBr3NPs is extremely low and estimated to be only 0.4 mW (0.126 MW cm−2). Finally, an ultrahigh lateral resolution of 20.6 nm is obtained for a single nanoparticle under 27.5 mW STED laser irradiation in CsPbBr3‐based STED nanoscopy imaging, which is a tenfold improvement compared with confocal microscopy. Because of its high fluorescence stability and ultrahigh resolution under lower depletion power, CsPbBr3‐assisted STED nanoscopy has great potential to investigate microstructures that require super‐resolution and long‐term imaging.