Amino Acid- and Growth Factor-Based Multifunctional Nanocapsules for the Modulation of the Local Microenvironment in Tissue Engineering.
Amino Acid- and Growth Factor-Based Multifunctional Nanocapsules for the Modulation of the Local Microenvironment in Tissue Engineering.
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DOI：
10.1021/acsami.0c15133
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发表时间：
2021-01
影响因子：
9.5
通讯作者：
Qijuan Yuan;Jun Huang;Caihong Xian;Jun Wu
中科院分区：
文献类型：
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作者：
Qijuan Yuan;Jun Huang;Caihong Xian;Jun Wu
伤口部位代谢物对细胞的氧化损伤是抑制组织再生的最棘手的因素之一，尤其是在大块损伤的情况下。此外，身体在伤口部位的过度炎症反应会使情况变得更糟。如何有效地抑制机体代谢和炎症反应产生的活性氧已成为组织工程研究的重要课题。在这里，我们利用天然生物活性小分子L-精氨酸和L-苯丙氨酸和生长因子肌醇合成支化聚（酯酰胺）（BPEA），制造BPEA纳米胶囊在伤口部位的维生素E输送。维生素E负载BPEA纳米胶囊（BPEA@VE NCs）可通过清除ROS保护细胞免受细胞内外损伤。同时，炎症反应也可以下调，受益于l-精氨酸的引入。此外，BPEA的生物降解产物是人体的天然代谢产物，如氨基酸和生长因子，保证了BPEA@VE NC的生物相容性。还在体内研究了BPEA@VE NC对加速伤口愈合的保护能力。所有结果表明，BPEA@VE NCs具有良好的抗氧化和抗炎特性，在组织工程中的局部微环境的调节有前途的潜力。
Oxidative damage to cells from metabolites at a wound site is one of the trickiest factors inhibiting tissue regeneration, especially with bulk damage. In addition, an excessive inflammatory reaction by the body at the wound site can make it even worse. How to scavenge the reactive oxygen species (ROS) produced from metabolism and inflammatory reactions has become a critical issue in tissue engineering. Here, we utilize the natural bioactive small molecules l-arginine and l-phenylalanine and the growth factor inositol to synthesize a branched poly(ester amide) (BPEA) to fabricate BPEA nanocapsules for vitamin E delivery at wound sites. BPEA nanocapsules loaded with vitamin E (BPEA@VE NCs) could protect cells from both extracellular and intracellular damage by scavenging ROS. Simultaneously, the inflammatory reaction could also be downregulated, benefiting from the introduction of l-arginine. Furthermore, the biodegradation products of BPEA are natural metabolites of the body, such as amino acids and growth factors, guaranteeing the biocompatibility of the BPEA@VE NCs. The protective ability of the BPEA@VE NCs was also investigated in vivo for accelerated wound healing. All the results indicate that the BPEA@VE NCs have promising potential for the modulation of the local microenvironment in tissue engineering for excellent antioxidative and anti-inflammatory properties.