Association between Group 2 Innate Lymphoid Cells enrichment, nasal polyps and allergy in Chronic Rhinosinusitis
Association between Group 2 Innate Lymphoid Cells enrichment, nasal polyps and allergy in Chronic Rhinosinusitis
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DOI：
10.1111/all.12440
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发表时间：
2014-09-01
期刊：
影响因子：
12.4
通讯作者：
Vreugde, S.
中科院分区：
文献类型：
--
作者：
Miljkovic, D.;Bassiouni, A.;Vreugde, S.
背景：在变态反应性疾病动物模型中，第2组天然淋巴样细胞(ILC2s)参与Th2型免疫反应的启动和协调。最近，在慢性鼻-鼻窦炎(CRS)患者中发现了ILC2s的丰富，然而，ILC2s在CRS中协调Th2反应中的作用仍不清楚。在此，我们通过研究CRS患者ILC2、Th2细胞和Th2细胞因子表达的相关性来表征CRS的ILC2亚群。方法：采用流式细胞术对29例CRS患者和5名对照组的鼻腔粘膜组织进行ILC2和Th2细胞的定量检测。采用定量逆转录聚合酶链式反应(QRT-PCR)检测鼻息肉组织中IL-5、IL-13、IL-25、IL-33、TSLP和GATA3的信使RNA表达水平。多元线性回归显示ILC2数与CRSwNP和过敏性CRS显著正相关，与既往鼻内窥镜手术次数负相关。组2先天淋巴细胞数量与Th2细胞频率显著相关。与对照组相比，CRSwNP患者IL-5和IL-13的信使RNA表达水平显著升高，而IL-25和GATA3的信使RNA水平显著降低。结论：CRS患者ILC2与其他Th2反应元件之间存在复杂的相互作用，提示CRSwNP和过敏性CRS患者存在ILC2的丰富。
Background: Group 2 innate lymphoid cells (ILC2s) were shown to be involved in the initiation and coordination of Th2-type immune responses in allergic disease animal models. Recently, ILC2s enrichment was noted in chronic rhinosinusitis (CRS) patients; however, the role of ILC2s in coordinating the Th2 response in CRS remains to be elucidated. Here, we characterize the ILC2 compartment in CRS by investigating the correlations between ILC2s, Th2 cells and Th2 cytokines expression in CRS patients.Methods: We used flow cytometric analysis of sinonasal mucosal tissues of 29 CRS patients and 5 controls to quantify ILC2s and Th2 cells. Messenger RNA expression levels of IL-5, IL-13, IL-25, IL-33, TSLP and GATA3 were determined using qRT-PCR.Results: ILC2s were significantly enriched in nasal polyps (CRSwNP) patients. Multivariate linear regression showed a significant positive association of ILC2 numbers with CRSwNP and allergic CRS and a negative association with the number of previous endoscopic sinus surgeries. Group 2 innate lymphoid cell numbers significantly correlated with Th2 cell frequencies. Messenger RNA expression levels of IL-5 and IL-13 were increased in CRSwNP compared with controls, while mRNA levels of IL-25 and GATA3 were significantly reduced.Conclusions: Our results characterize the complex interactions between ILC2s and other Th2 response elements in the context of CRS and suggest that ILC2 enrichment occurs in CRSwNP and in allergic CRS patients.