Identification of the biosynthetic units of Cypridina luciferin in Cypridina (Vargula) hilgendorfii by LC/ESI-TOF-MS
Identification of the biosynthetic units of Cypridina luciferin in Cypridina (Vargula) hilgendorfii by LC/ESI-TOF-MS
复制标题
DOI：
10.1016/j.tet.2004.09.080
复制
发表时间：
2004-12-06
期刊：
影响因子：
2.1
通讯作者：
Inouye, S
中科院分区：
文献类型：
--
作者：
Kato, S;Oba, Y;Inouye, S
在发光介形虫Cypridina（Vargula）hilgendorfii中，具有咪唑并吡嗪酮结构（3，7-二氢咪唑并吡嗪-3-酮）的Cypridina pectin用于发光反应。为了确定Cypridina的生物合成单位，稳定同位素标记的化合物进行了检查与生活Cypridina标本饲养实验。通过LC/ESI-TOF-MS分析证实了标记化合物掺入到Cypridina pellin中，这些结果表明L-色氨酸、L-精氨酸和L-异亮氨酸是Cypridina pellin的结构单元。(C)2004爱思唯尔有限公司保留所有权利。
In a luminous ostracod Cypridina (Vargula) hilgendorfii, Cypridina luciferin with an imidazopyrazinone structure (3,7-dihydroimidazopyrazin-3-one) is utilized for the luminescence reaction. To identify the biosynthetic units of Cypridina luciferin, the stable isotope labeled compounds were examined by feeding experiments with living Cypridina specimens. The incorporation of the labeled compounds into Cypridina luciferin was identified by the method of LC/ESI-TOF-MS analyses and these results suggested that L-tryptophan, L-arginine and L-isoleucine are structural units of Cypridina luciferin. (C) 2004 Elsevier Ltd. All rights reserved.