Functional arteries grown in vitro
Functional arteries grown in vitro
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DOI：
10.1126/science.284.5413.489
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发表时间：
1999-04-16
期刊：
影响因子：
56.9
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Niklason, LE;Gao, J;Langer, R
开发了一种组织工程方法，用于从血管组织活检获得的平滑肌和内皮细胞中制备任意长度的血管移植物材料。在脉动条件下培养的牛血管的破裂强度大于2000毫米汞柱，缝线保留强度高达90克，胶原蛋白含量高达50%。培养的血管也表现出对药物的收缩反应，并含有显示分化标志物（如钙调蛋白和肌球蛋白重链）的平滑肌细胞。将组织工程化动脉植入小型猪体内，通过数字血管造影术记录了长达24天的通畅性。
A tissue engineering approach was developed to produce arbitrary lengths of vascular graft material from smooth muscle and endothelial cells that were derived from a biopsy of vascular tissue. Bovine vessels cultured under pulsatile conditions had rupture strengths greater than 2000 millimeters of mercury, suture retention strengths of up to 90 grams, and collagen contents of up to 50 percent. Cultured vessels also showed contractile responses to pharmacological agents and contained smooth muscle cells that displayed markers of differentiation such as calponin and myosin heavy chains. Tissue-engineered arteries were implanted in miniature swine, with patency documented up to 24 days by digital angiography.