Recent Developments in Biomolecular NMR
Recent Developments in Biomolecular NMR
复制标题
生物分子核磁共振的最新进展
DOI：
10.1039/9781849735391-00271
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Bostock M
中科院分区：
文献类型：
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作者：
Bostock M
据预测，膜蛋白约占基因组中蛋白质的三分之一，1然而，迄今为止，它们在结构上仍显着不足，其独特结构仅占所有PDB沉积结构的0.4%。2，3然而，这些数字必须与最近膜蛋白（包括哺乳动物蛋白）结构的激增相对照，4遵循预测的指数趋势。虽然这些结构中的许多已经通过X射线晶体学解决，但NMR也贡献了相当大的数量，7目前为16%。8在固态NMR领域也取得了相当大的进展，9-11然而，在这篇综述中，我们专注于溶液态NMR。特别是，一些最近的结构表明，解决方案NMR能够解决一系列大的膜蛋白高达约100 kDa。b-Barrel蛋白12 -15仍然更容易研究，因为它们的二级结构允许通过相邻链之间的氢键和短程NOE获得取向信息。最近，NMR也被证明能够研究一系列大的α-螺旋膜蛋白，16-19表明该技术已经成熟到可以认为通过NMR确定膜蛋白结构是可行方法的水平。
Membrane proteins are predicted to make up approximately one-third of proteins in the genome, 1 however, to date they remain significantly structurally under-represented, with unique structures comprising only 0.4% of all PDB-deposited structures. 2, 3 Nevertheless these figures must be set against a recent surge in structures of membrane proteins, including mammalian proteins, 4 following a predicted exponential trend. 5, 6 Whilst many of these structures have been solved by X-ray crystallography, NMR has also contributed a significant number, 7 currently 16%. 8 Considerable progress has also been made in the field of solid-state NMR, 9–11 however, in this review we focus on solution-state NMR. In particular, a number of recent structures demonstrate that solution NMR is able to tackle a range of large membrane proteins up to around y100 kDa. b-Barrel proteins12–15 remain easier to study because their secondary structure allows orientational information to be obtained through hydrogen bonds and short-range NOEs between adjacent strands. Recently, NMR has also proved able to study a range of large a-helical membrane proteins, 16–19 demonstrating that the technology has matured to a level at which membrane protein structure determination by NMR can be considered a viable approach.