Structural variability of endotoxins from R-type isogenic mutants of Shigella sonnei
Structural variability of endotoxins from R-type isogenic mutants of Shigella sonnei
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DOI：
10.1002/jms.1863
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发表时间：
2011-01-01
影响因子：
2.3
通讯作者：
Kilar, Ferenc
中科院分区：
文献类型：
--
作者：
Kilar, Aniko;Doernyei, Agnes;Kilar, Ferenc
宋内氏志贺菌突变株粗型内毒素[脂多糖（LPS）]的结构变异。采用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）和串联质谱（TMS-MS）技术对一系列S. Sonnei突变体以前是对LPS核心寡糖区域中庚糖组分生物合成的分析研究的对象。本研究对S.结果表明，粗蛋白突变体的LPS呈阶梯状形成，在4350、562 H、R41和4303的深层核心区分别含有0：1：2：3的庚糖，其中粗蛋白突变体的LPS最长。Sonnei 4303还含有五种己糖。随后通过MALDI-TOF-MS/MS对完整LPS的脂质A部分和寡糖部分的结构变化进行分析。对于样品的溶解和电离，将柠檬酸溶液中的2，5-二羟基苯甲酸用作基质。质谱的详细评价表明，由于磷酸盐和脂肪酸组成的差异，脂质部分存在异质性。版权所有（C）2010约翰威利父子有限公司
The structural variations in the rough-type endotoxins [lipopolysaccharides (LPSs)] of Shigella sonnei mutant strains (S. sonnei phase II - 4303, R41, 562H and 4350) were investigated by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem MS. A series of S. sonnei mutants had previously been the subject of analytical studies on the biosynthesis of heptose components in the core oligosaccharide region of LPSs. This study gives a complete overview on the structures of the full core and lipid A of S. sonnei mutant strains by MS. We found that the LPSs of the isogenic rough mutants were formed in a step-like manner containing 0: 1 : 2 : 3 heptose in the deep core region of 4350, 562H, R41 and 4303, respectively, and the longest LPS from the mutant S. sonnei 4303 contained also five hexoses. The structural variations in the lipid A moiety and in the oligosaccharide part of the intact LPS were followed by MALDI-TOF-MS/MS. For the dissolution and the ionization of the samples, 2,5-dihydroxybenzoic acid in citric acid solution was applied as matrix. The detailed evaluation of the mass spectra indicates heterogeneity in the lipid part due to the differences in the phosphate and fatty acid composition. Copyright (C) 2010 John Wiley & Sons, Ltd.