DMSO Induces Dehydration near Lipid Membrane Surfaces
DMSO Induces Dehydration near Lipid Membrane Surfaces
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DOI：
10.1016/j.bpj.2015.06.011
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发表时间：
2015-07-21
影响因子：
3.4
通讯作者：
Han, Songi
中科院分区：
文献类型：
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作者：
Cheng, Chi-Yuan;Song, Jinsuk;Han, Songi
二甲基亚砜（DMSO）作为助溶剂、低温保护剂和膜通透性增强剂在生物学中得到了广泛的应用，人们普遍认为DMSO诱导的细胞膜结构变化及其水合水在生物学中起着重要的功能作用。虽然DMSO对膜结构和头群脱水的影响已被广泛研究，但DMSO对脂质膜的影响机制以及水在这一过程中的直接作用尚未明确。通过直接探测无约束脂质囊泡表面附近的平移水扩散率、脂质头基团迁移率和多层囊泡中的重复距离，我们发现DMSO在体积DMSO摩尔分数（X-DMSO）的情况下，只会削弱脂质膜附近的地表水网络
Dimethyl sulfoxide (DMSO) has been broadly used in biology as a cosolvent, a cryoprotectant, and an enhancer of membrane permeability, leading to the general assumption that DMSO-induced structural changes in cell membranes and their hydration water play important functional roles. Although the effects of DMSO on the membrane structure and the headgroup dehydration have been extensively studied, the mechanism by which DMSO invokes its effect on lipid membranes and the direct role of water in this process are unresolved. By directly probing the translational water diffusivity near unconfined lipid vesicle surfaces, the lipid headgroup mobility, and the repeat distances in multilamellar vesicles, we found that DMSO exclusively weakens the surface water network near the lipid membrane at a bulk DMSO mole fraction (X-DMSO) of