Surface Modification of Halloysite Nanotubes with Dopamine for Enzyme Immobilization
Surface Modification of Halloysite Nanotubes with Dopamine for Enzyme Immobilization
复制标题
多巴胺对埃洛石纳米管进行表面修饰用于酶固定化
DOI：
10.1021/am4022973
复制
发表时间：
2013-11-13
影响因子：
9.5
通讯作者：
Chen, Rongfeng
中科院分区：
文献类型：
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作者：
Chao, Cong;Liu, Jindun;Chen, Rongfeng
埃洛石纳米管（HNTs）已被提议作为一种潜在的支持酶。提高酶在HNT上的负载量对其实际应用至关重要。在此，我们报道了一种简单的方法，通过在HNTs表面修饰多巴胺来制备高载酶载体。通过透射电子显微镜、傅里叶变换红外光谱和X射线光电子能谱对改性后的HNTs进行了表征。结果表明，多巴胺能自组装附着在HNTs表面，形成一层薄薄的活性涂层。将所制备的杂化纳米管用于漆酶的负载，负载量达到168.8mg/g载体，大大高于纯HNTs的负载量（11.6mg/g载体）。固定化漆酶贮存30天后仍能保持90%以上的初始酶活，而游离漆酶的初始酶活仅为32%。固定化漆酶经5次重复使用后仍能保持90%以上的初始酶活。此外，固定化漆酶对酚类化合物的降解速度快，降解效率高。这些优点表明，这种新的杂化材料可以作为一种低成本、有效的载体来抑制酶的活性。
Halloysite nanotubes (HNTs) have been proposed as a potential support to immobilize enzymes. Improving enzyme loading on HNTs is critical to their practical applications. Herein, we reported a simple method on the preparation of high-enzyme-loading support by modification with dopamine on the surface of HNTs. The modified HNTs were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy analyses. The results showed that dopamine could self-polymerize to adhere to the surface of HNTs and form a thin active coating. While the prepared hybrid nanotubes were used to immobilize enzyme of laccase, they exhibited high loading ability of 168.8 mg/g support, which was greatly higher than that on the pristine HNTs (11.6 mg/g support). The immobilized laccase could retain more than 90% initial activity after 30 days of storage and the free laccase only 32%. The immobilized laccase could also maintain more than 90% initial activity after five repeated uses. In addition, the immobilized laccase exhibited a rapid degradation rate and high degradation efficiency for removal of phenol compounds. These advantages indicated that the new hybrid material can be used as a low-cost and effective support to immobilize enzymes.