SILVER IMPREGNATION OF ALZHEIMER NEUROFIBRILLARY CHANGES COUNTERSTAINED FOR BASOPHILIC MATERIAL AND LIPOFUSCIN PIGMENT
SILVER IMPREGNATION OF ALZHEIMER NEUROFIBRILLARY CHANGES COUNTERSTAINED FOR BASOPHILIC MATERIAL AND LIPOFUSCIN PIGMENT
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DOI：
10.3109/10520298809107184
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发表时间：
1988-07-01
期刊：
影响因子：
--
通讯作者：
BOHL, J
中科院分区：
文献类型：
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作者：
BRAAK, H;BRAAK, E;BOHL, J
本文描述了一种方法，其中阿尔茨海默氏病神经病理学变化的选择性银染色与细胞核、尼氏物质和脂褐质颗粒的染色相结合。根据Gallyas提出的方法，将福尔马林固定、石蜡包埋的人尸检组织切片进行银染色。过甲酸氧化后，脂褐素被巴豆醛品红染色。尼氏物质通过达罗红或没食子花青-铬明矾染色可见。使用这种技术可以识别出显示特定病理的结构单元和易于发生神经病理变化的神经元类型。
A method is described in which selective silver staining of Alzheimer''s neurofibrillary changes is combined with staining of cell nuclei, Nissl material, and lipofuscin granules. Formalin fixed, paraffin embedded sections of human autopsy tissue are silver stained according to a method proposed by Gallyas. Lipofuscin is stained by crotonaldehyde fuchsin following performic acid oxidation. Nissl substance is visualized by either Darrow red or gallocyanin-chrome alum staining. Architectonic units showing the specific pathology and the neuronal types prone to develop the neurofibrillary changes can be recognized using this technique.