Primary rat hepatocytes express cyclin D1 messenger RNA during their growth cycle and during mitogenic transitions induced by transforming growth factor-alpha.
Primary rat hepatocytes express cyclin D1 messenger RNA during their growth cycle and during mitogenic transitions induced by transforming growth factor-alpha.
复制标题
原代大鼠肝细胞在生长周期和转化生长因子-α 诱导的有丝分裂转变期间表达细胞周期蛋白 D1 信使 RNA。
DOI：
10.1006/bbrc.1994.2430
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
Leffert,HL
中科院分区：
文献类型：
--
作者：
Koch,KS;Lu,XP;Leffert,HL
Cyclin D1 messenger RNA expression was investigated in differentiated proliferation-competent primary cultures of adult rat hepatocytes. Periodic expression was observed during the 12-day growth cycle (≍two population doublings). Upon reaching a quiescent G0-state, 4-kb cyclin D1 mRNA levels were undetectable. When G0cultures were shifted into defined media without or with 0.2-0.8 nM TGF-α, conditions that reinitiate full proliferative transitions synchronously, cyclin D1 mRNA levels were elevated 1.2-4.6-fold, respectively. These findings support the hypothesis that hepatic mitogens stimulate periodic cyclin mRNA expression directly.
