Expression of superficial zone protein in mandibular condyle cartilage
Expression of superficial zone protein in mandibular condyle cartilage
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DOI：
10.1016/j.joca.2006.02.002
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发表时间：
2006-08-01
影响因子：
7
通讯作者：
Knudson, W.
中科院分区：
文献类型：
--
作者：
Ohno, S.;Schmid, T.;Knudson, W.
目的：表面区蛋白(SZP)在四肢关节软骨的边界润滑中起作用。然而，SZP在下颌软骨中的表达尚不清楚，下颌软骨是一种表面含有厚厚纤维层的组织。本研究旨在阐明SZP在下颌骨组织中的分布及体液因子对其在外植体和成纤维细胞中表达的调节作用。方法：测定SZP在牛下颌骨软骨中的分布，观察SZP在组织中的表达及转化生长因子-β(TGF-β)对SZP表达的影响。在外植体和培养成纤维细胞中，转化生长因子-β均上调SZP的表达，而IL-1β则轻微下调SZP的表达。经转化生长因子-β处理的成纤维细胞培养上清液中SZP蛋白的积聚也显著增加。结论：SZP在下颌骨髁状突软骨的边界润滑中起重要作用，在其内部局部合成，并受与下颌骨髁状突修复和病理相关的细胞介质的差异性调节。(C)2006年国际骨性关节炎研究会。爱思唯尔有限公司出版。保留所有权利。
Objective: Superficial zone protein (SZP) has been shown to function in the boundary lubrication of articular cartilages of the extremities. However, the expression of SZP has not been clarified in mandibular cartilage which is a tissue that includes a thick fibrous layer on the surface. This study was conducted to clarify the distribution of SZP on the mandibular condyle and the regulatory effects of humoral factors on the expression in both explants and fibroblasts derived from mandibular condyle.Methods: The distribution of SZP was determined in bovine mandibular condyle cartilage, and the effects of interleukin-1 beta (IL-1 beta) and transforming growth factor-beta (TGF-beta) on SZP expression were examined in condyle explants and fibroblasts derived from the fibrous zone of condyle cartilage.Results: SZP was highly distributed in the superficial zone of intact condyle cartilage. The SZP expression was up-regulated by TGF-beta in both explants and cultured fibroblasts, whereas the expression was slightly down-regulated by IL-1 beta. A significant increase in accumulation of SZP protein was also observed in the culture medium of the fibroblasts treated with TGF-beta.Conclusions: These results suggest that SZP plays an important role in boundary lubrication of mandible condylar cartilage, is synthesized locally within the condyle itself, and exhibits differential regulation by cell mediators relevant to mandibular condyle repairing and pathologies. (C) 2006 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.