The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth
The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth
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DOI：
10.1007/s00784-009-0261-9
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发表时间：
2010-02-01
影响因子：
3.4
通讯作者：
Higham, S. M.
中科院分区：
文献类型：
--
作者：
Adeyemi, A. A.;Jarad, F. D.;Higham, S. M.
本研究报告了一种新的方法，使用定量光诱导荧光（QLF）的开发和评估，使其能够用于量化和评估整个牙齿表面染色和牙齿美白。将该方法与分光光度计进行比较，以评估可靠性。对16颗离体牛牙进行了两个实验阶段，即内在着色形成和牙齿美白。使用茶溶液（2g/100 ml，Marks and Spencer Extra Strong Tea，Marks and Spencer，伦敦，英国）通过这些牙齿的舌面和根管进入，持续6天，形成内在着色。使用33%过氧化氢（VWR Prolab，Leicestershire，UK）在150分钟内循环去除污渍。使用QLF（Inspektor Research systems，Amsterdam，Netherlands）和分光光度法（Easy shade，Vita Zahnfabrik，Bad Sackingen，德国）监测污渍形成/变白。获得了QLF的参数Δ F和分光光度计的参数Δ E。使用Pearson相关系数测试通过所述方法观察到的污渍强度和去除的进展的相关性。检验了每种方法的检查者内部可靠性。QLF与分光光度法在检测和监测牙本质着色进展方面显示出高度相关性（Pearson系数r为-0.987，相关性显著p < 0.0001）。对于去污，两种方法之间的皮尔逊系数（r）为-0.906，无显著性p = 0.094。使用外部参考材料结合内部贴片QLF分析技术能够检测和测量整个牙齿表面染色及其纵向去除。该方法的可靠性显示了潜在的临床应用价值。
This study reports the development and evaluation of a novel method using quantitative light-induced fluorescence (QLF), which enables its use for quantifying and assessing whole tooth surface staining and tooth whitening. The method was compared with a spectrophotometer to assess reliability. Two experimental phases, intrinsic stain formation and tooth whitening, were conducted in vitro on 16 extracted bovine teeth. Intrinsic stains were developed via access through lingual surfaces and root canals of these teeth using tea solution (2 g/100 ml, Marks and Spencer Extra Strong Tea, Marks and Spencer, London, UK) for 6 days. Stains were removed using 33% hydrogen peroxide (VWR Prolab, Leicestershire, UK) in cycles over 150 min. Stain development/whitening was monitored with QLF (Inspektor Research systems, Amsterdam, Netherlands) and spectrophotometry (Easy shade, Vita Zahnfabrik, Bad Sackingen, Germany). Parameters Delta F for QLF and Delta E for the spectrophotometer were obtained. The progression of stain intensity and removal observed by the methods were tested for correlation using Pearson's correlation coefficient. Intra-examiner reliability for each method was tested. QLF showed a high correlation with spectrophotometry for detecting and monitoring intrinsic tooth stain progression (Pearson coefficient r was -0.987 with correlation significant p < 0.0001). For stain removal, the Pearson coefficient (r) between both methods was -0.906 with no significance p = 0.094. The use of an external reference material in combination with the inner patch QLF analysis technique had the ability to detect and measure whole tooth surface staining and its removal longitudinally. The reliability of the method shows a potential clinical application.