A Systems Biology Approach to Characterize Biomarkers for Blood Stasis Syndrome of Unstable Angina Patients by Integrating MicroRNA and Messenger RNA Expression Profiling.
A Systems Biology Approach to Characterize Biomarkers for Blood Stasis Syndrome of Unstable Angina Patients by Integrating MicroRNA and Messenger RNA Expression Profiling.
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DOI：
10.1155/2013/510208
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Yu G
中科院分区：
文献类型：
--
作者：
Wang J;Yu G
血液暂停综合征（BSS）被认为是不稳定心绞痛（UA）患者的主要综合征类型。这项研究的目的是为UA的BSS找到基于系统生物学的microRNA（miRNA）和mRNA表达生物标志物。我们通过微阵列确定了在UA患者的BS和健康对照组之间差异表达的1081个mRNA和25个miRNA。我们使用David，mirtrail和蛋白质 - 蛋白质相互作用方法来探索差异表达的miRNA和mRNA的相关途径和网络。通过结合途径和网络的结果，我们发现miR-146b-5p的上调可能诱导CALR下调减轻炎症，而miR-199a-5p的上调可能会诱导TP53的下调，以抑制BSS的凋亡。 UA患者。 MiR-146b-5p，miR-199a-5p，Calr和TP53的表达模式通过QRT-PCR在一个独立的验证队列中证实，包括UA患者的BB，UA患者的非BBS，UA患者的非BBS和健康对照组。 miR-146b-5p，miR-199a-5p，Calr和TP53可能是UA患者BS的重要生物标志物。在决定干预措施或临床试验时，基于系统生物学的miRNA和mRNA表达生物标志物可能有助于UA患者的进一步分层。
Blood stasis syndrome (BSS) has been considered to be the major type of syndromes in unstable angina (UA) patients. The aim of this study was to find the systems biology-based microRNA (miRNA) and mRNA expression biomarkers for BSS of UA. We identified 1081 mRNAs and 25 miRNAs differentially expressed between BSS of UA patients and healthy controls by microarrays. We used DAVID, miRTrail, and the protein-protein interactions method to explore the related pathways and networks of differentially expressed miRNAs and mRNAs. By combining the results of pathways and networks, we found that the upregulation of miR-146b-5p may induce the downregulation of CALR to attenuate inflammation and the upregulation of miR-199a-5p may induce the downregulation of TP53 to inhibit apoptosis in BSS of UA patients. The expression patterns of miR-146b-5p, miR-199a-5p, CALR, and TP53 were confirmed by qRT-PCR in an independent validation cohort including BBS of UA patients, non-BBS of UA patients, and healthy controls. miR-146b-5p, miR-199a-5p, CALR, and TP53 could be significant biomarkers of BSS of UA patients. The systems biology-based miRNA and mRNA expression biomarkers for the BSS of UA may be helpful for the further stratification of UA patients when deciding on interventions or clinical trials.
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