The effect of transplantation of nasal mucosal epithelial cell sheets after middle ear surgery in a rabbit model
The effect of transplantation of nasal mucosal epithelial cell sheets after middle ear surgery in a rabbit model
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DOI：
10.1016/j.biomaterials.2014.11.037
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发表时间：
2015-02-01
期刊：
影响因子：
14
通讯作者：
Kojima, Hiromi
中科院分区：
文献类型：
--
作者：
Yamamoto, Kazuhisa;Hama, Takanori;Kojima, Hiromi
中耳手术后的中耳粘膜再生和中耳腔的再造是非常重要的。本研究建立了一种将自体鼻粘膜上皮细胞片移植到受损中耳腔的新方法。本研究的目的是评估细胞片移植后的术后愈合情况。将兔鼻粘膜上皮细胞片在温度敏感培养皿上制备，并移植到手术造成的兔中耳损伤处。术后组织学和X线计算机断层扫描评价中耳愈合情况。通过测量反映跨粘膜气体交换功能的最大中耳总压进行功能评价。采用两个对照组：正常对照组和去粘膜对照组。与去黏膜对照组相比，细胞片移植可抑制中耳腔骨质增生和气腔狭窄。粘膜气体交换功能在细胞片移植组中也更好。鼻粘膜上皮细胞片被证实是一种有效的中耳手术后移植材料，并有望成为一种新的治疗方法。(C)2014爱思唯尔有限公司版权所有。
Postoperative regeneration of the middle ear mucosa and pneumatization of the middle ear cavity are of great importance after middle ear surgery. This study developed a new method to transplant autologous nasal mucosal epithelial cell sheets into the damaged middle ear cavity. The aim of this study was to evaluate postoperative healing after the transplantation of the cell sheets. Rabbit nasal mucosal epithelial cell sheets were fabricated on a temperature-responsive culture dish, and transplanted into the damaged middle ear of rabbit, which was surgically created. The healing of middle ears was evaluated by histology and X-ray computed tomography after transplantation. Functional evaluation was performed by measuring the maximum middle ear total pressure reflecting a trans-mucosal gas exchange function. Two control groups were used: the normal control group and the mucosa-eliminated control group. Transplantation of cell sheets suppressed the bone hyperplasia and the narrowing of pneumatic space in the middle ear cavity compared with the mucosa-eliminated control group. The mucosal gas exchange function was also better in the cell sheet-transplanted group. Nasal mucosal epithelial cell sheet was confirmed to be useful as an effective graft material after middle ear surgery and hopefully become a novel therapy in the future. (C) 2014 Elsevier Ltd. All rights reserved.