Single-step analysis of individual conjugated bile acids in human bile using 1H NMR spectroscopy
Single-step analysis of individual conjugated bile acids in human bile using 1H NMR spectroscopy
复制标题
DOI：
10.1007/s11745-006-5008-7
复制
发表时间：
2006-06-01
期刊：
影响因子：
1.9
通讯作者：
Khetrapal, C. L.
中科院分区：
文献类型：
--
作者：
Gowda, G. A. Nagana;Ijare, Omkar B.;Khetrapal, C. L.
使用一维（H-1和C-13）和二维（H-1-H-1和H-1-C-13）实验对完整人胆汁的H-1和C-13 NMR谱进行了归属。鉴别了单个结合胆汁酸-甘氨胆酸、甘氨脱氧胆酸、甘氨鹅脱氧胆酸、牛磺胆酸、牛磺脱氧胆酸和牛磺鹅脱氧胆酸。通过使用独特的和特征性的酰胺信号，在一个步骤中准确和单独地定量胆汁酸。利用C-13 NMR，该研究还提出了一种单独分析未结合胆汁酸（如果存在）的方法。本文所述的完整胆汁中单个结合胆汁酸的化学位移分配和快速单步分析可能在胆汁酸代谢的研究和肝胆疾病的更深入理解中具有巨大的实用性。
H-1 and C-13 NMR spectra of intact human bile were assigned using one-dimensional (H-1 and C-13) and two-dimensional (H-1-H-1 and H-1-C-13) experiments. Individual conjugated bile acids-glycocholic acid, glycodeoxycholic acid, glycochenodeoxycholic acid, taurocholic acid, taurodeoxycholic acid, and taurochenodeoxycholic acid-were identified. The bile acids were quantified accurately and individually in a single step by using distinct and characteristic amide signals. Making use of C-13 NMR, the study also suggests a way to analyze unconjugated bile acids separately, if present. Chemical shift assignments and rapid single-step analysis of individual conjugated bile acids from intact bile presented herein may have immense utility in the study of bile acid metabolism and deeper understanding of hepatobiliary diseases.