Expressions of vascular endothelial growth factor and basic fibroblast growth factor in tumors induced by two different cloned cell lines established from transplantable rat malignant fibrous histiocytoma
Expressions of vascular endothelial growth factor and basic fibroblast growth factor in tumors induced by two different cloned cell lines established from transplantable rat malignant fibrous histiocytoma
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DOI：
10.1292/jvms.62.699
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发表时间：
2000-07-01
影响因子：
1.2
通讯作者：
Sakuma, S
中科院分区：
文献类型：
--
作者：
Tsunenari, I;Yamate, J;Sakuma, S
为探讨血管生成因子在间叶细胞瘤发生发展中的作用，采用免疫组织化学和Western blotting方法，检测了F344大鼠移植性恶性纤维组织细胞瘤（MFH）MT-8和MT-9移植瘤中血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的表达。MT-8和MT-9肿瘤通过植入肿瘤组织碎片在同系大鼠中形成。在植入后（PI）第3、6、9和17天检查MT-8肿瘤，并且在PI第9、14、17和23天检查MT-9肿瘤。MT-8肿瘤的生长速度比MT-9肿瘤的生长速度快。组织学上，MT-8肿瘤是未分化肉瘤的特征，而MT-9肿瘤表现出典型的MFH的故事状生长模式。免疫组化显示，构成MT-8和MT-9肿瘤的所有细胞均与VEGF和bFGF抗体发生反应，表明间充质肿瘤细胞产生这些因子。然而，这些免疫反应在所检查的特纳之间没有显著差异。因此，对Western印迹法中获得的条带进行光密度扫描。MT-8肿瘤中VEGF和bFGF的表达水平在PI第3至9天逐渐增加，MT-9肿瘤中PI第9至17天逐渐增加。在最后检查日，两种肿瘤中bFGF和MT-9肿瘤中VEGF的表达水平均下降，但MT-8肿瘤中VEGF的表达水平仍升高。结果提示VEGF和bFGF在间充质干细胞发育早期协同促进血管生成。
In order to establish base-line data on angiogenic factors in development of mesenchymal tumors, expressions of vascular endothelial growth factor (VEGF) and basic Fibroblast growth factor (bFGF) in implanted MT-8 and MT-9 tumors, both derived From a transplantable malignant fibrous histiocytoma (MFH) in the F344 rat, were investigated by immunohistochemistry and Western blotting method. MT-8 and MT-9 tumors were developed in syngeneic rats by implant of a tumor tissue fragment. MT-8 tumors were examined on post-implantation (PI) days 3, 6, 9 and 17, and MT-9 tumors were on PI days 9, 14, 17 and 23. The growth of MT-8 tumors was faster than that of MT-9 turners. Histologically, MT-8 tumors were features of undifferentiated sarcomas, whereas MT-9 tumors exhibited a typical storiform growth pattern of MFH. Immunohistochemically, all cells constituting MT-8 and MT-9 tumors reacted with antibodies to VEGF and bFGF, indicating production of these Factors by mesenchymal neoplastic cells. However, there were no marked differences in these immunoreactions between turners examined. Thus, the bands obtained in the Western blotting methods were densitometrically scanned. The expression levels of VEGF and bFGF gradually increased PI day 3 to 9 in MT-8 tumors and PI day 9 to 17 in MT-9 tumors. On last examination day, the levels of bFGF in both tumors and of VEGF in MT-9 tumors decreased, but the VEGF expression level in MT-8 turners was still increased. Tnt se findings indicated that VEGF and bFGF may contribute cooperatively to angiogenesis in an early growth of mesenchymal turner development.