Susceptibility of the tomato mutant high pigment-2dg (hp-2dg) to Orobanche spp. infection
Susceptibility of the tomato mutant high pigment-2dg (hp-2dg) to Orobanche spp. infection
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DOI：
10.1021/jf800760x
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发表时间：
2008-08-13
影响因子：
6.1
通讯作者：
Bouwmeester, Harro
中科院分区：
文献类型：
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作者：
Lopez-Raez, Juan Antonio;Charnikhova, Tatsiana;Bouwmeester, Harro
具有潜在健康益处的天然产品的消费量不断增长，增强色素沉着对水果和蔬菜具有重要的经济意义。番茄 hp-2(dg) 是一个重要的突变系，已被引入商业番茄品种中，作为富含番茄红素的番茄 (LRT) 销售，因为它们的果实色素沉着增强，这归因于更高水平的类胡萝卜素（包括番茄红素）。独脚金内酯是介导根部寄生植物中宿主寻找的信号化合物，是从类胡萝卜素生物合成而来的。考虑到hp-2(dg)突变体的高类胡萝卜素含量，我们研究了其对根寄生虫列当的敏感性。在田间实验中，与同基因野生型对应物相比，在 hp-2(dg) 上生长的埃及列当植物的平均数量出人意料地显着减少。体外测定和 LC-MS/MS 分析表明，这种减少与独脚金内酯产量的降低有关，这显然使高类胡萝卜素 hp-2(dg) 突变体对列当的敏感性降低。
The consumption of natural products with potential health benefits has been continuously growing, and enhanced pigmentation is of major economic importance in fruits and vegetables. The tomato hp-2(dg) is an important mutant line that has been introgressed into commercial tomato cultivars marketed as lycopene rich tomatoes (LRT) because of their enhanced fruit pigmentation, attributed to higher levels of carotenoids, including lycopene. Strigolactones are signaling compounds that mediate host finding in root parasitic plants and are biosynthetically derived from carotenoids. Considering the high carotenoid content of the hp-2(dg) mutant, we studied its susceptibility to the root parasite Orobanche. In a field experiment, the average number of Orobanche aegyptiaca plants growing on hp-2(dg) was surprisingly significantly reduced compared with its isogenic wild-type counterpart. In vitro assays and LC-MS/MS analysis showed that this reduction was associated with a lower production of strigolactones, which apparently renders the high-carotenoid hp-2(dg) mutant less susceptible to Orobanche.