Primary prevention of asthma: age and sex influence sensitivity to allergen-induced airway inflammation and contribute to asthma heterogeneity in Guinea pigs.
Primary prevention of asthma: age and sex influence sensitivity to allergen-induced airway inflammation and contribute to asthma heterogeneity in Guinea pigs.
复制标题
哮喘的一级预防：年龄和性别影响对过敏原引起的气道炎症的敏感性，并导致豚鼠哮喘的异质性。
DOI：
10.1159/000095294
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发表时间：
2006
影响因子：
2.8
通讯作者：
Mohrman,MargaretE
中科院分区：
文献类型：
--
作者：
Regal,JeanF;Regal,RonaldR;Meehan,JessicaL;Mohrman,MargaretE
背景：在致敏阶段限制过敏原暴露已被提出作为一种手段，一级预防哮喘，但其有效性是debated.Hypothesis：一级预防哮喘是更有效地限制哮喘症状的年轻豚鼠相比，成年人，无论是男性或females.Methods：以下实验组：年轻/年轻，致敏和性成熟前的挑战;年轻/成人，致敏的年轻和性成熟后的挑战;成人/成人，致敏和性成熟后的挑战。雄性和雌性动物用不同剂量的卵清蛋白（OVA）腹腔内致敏，并用恒定剂量的OVA进行肠道内攻击。细胞浸润到肺和灌洗液，以及气道高反应性静脉乙酰甲胆碱测定24 h后。结果：在未致敏动物，居民炎症细胞的密度以及基线肺功能不同的年龄和性别。最大的OVA诱导的嗜酸性粒细胞增多症的女性发生在一个较低的致敏剂量的OVA比男性，和性别之间的剂量反应关系的斜率显着不同。与一些成年给药组相比，年轻雌性动物的嗜酸性粒细胞增加更明显。OVA特异性抗体的浓度与细胞浸润的差异无直接关系。气道高反应性乙酰甲胆碱的挑战，观察在所有的治疗groups.Conclusion：年轻的动物需要主要减少过敏原暴露与成人相比，有效地限制气道炎症的一级预防。哮喘症状的年龄和性别异质性表明，通过限制过敏原暴露或抗炎或支气管扩张药物治疗进行一级预防可能是特定年龄和性别人群更有效的策略。
Background:Limiting allergen exposure in the sensitization phase has been proposed as a means of primary prevention of asthma, but its effectiveness is debated.Hypothesis:Primary prevention of asthma is more effective in limiting asthma symptoms in young guinea pigs compared with adults, whether males or females.Methods:The following experimental groups were used: young/young, sensitized and challenged before sexual maturity; young/adult, sensitized young and challenged after sexual maturity; adult/adult, sensitized and challenged after sexual maturity. Males and females were sensitized intraperitoneally with varying doses of ovalbumin (OVA) and challenged intratracheally with a constant OVA dose. Cellular infiltration into lung and lavage fluid as well as airway hyperresponsiveness to intravenous methacholine was determined 24 h later.Results:In unsensitized animals, density of resident inflammatory cells as well as baseline pulmonary function differed with age and sex. Maximum OVA-induced eosinophilia in females occurred at a lower sensitizing dose of OVA than in males, and the slopes of the dose-response relationship differed significantly between sexes. Young females had more pronounced increases in eosinophils compared with some adult treatment groups. The concentrations of OVA-specific antibodies were not directly related to differences in cellular infiltration. Airway hyperresponsiveness to methacholine challenge was observed in all treatment groups.Conclusion:Young animals require major reductions in allergen exposure compared with adults to effectively limit airway inflammation in primary prevention. Heterogeneity of asthma symptoms seen with age and sex suggests that primary prevention by limiting allergen exposure or treatment with anti-inflammatory or bronchodilator drugs may be more effective strategies for specific age and gender populations.