Clinical outcome of autologous chondrocyte implantation is correlated with infrared spectroscopic imaging-derived parameters
Clinical outcome of autologous chondrocyte implantation is correlated with infrared spectroscopic imaging-derived parameters
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DOI：
10.1016/j.joca.2012.05.007
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发表时间：
2012-09-01
影响因子：
7
通讯作者：
Pleshko, N.
中科院分区：
文献类型：
--
作者：
Hanifi, A.;Richardson, J. B.;Pleshko, N.
目的：探讨傅立叶变换红外成像光谱分析(FT-IRIS)是否可以替代组织学和免疫组织化学(IHC)来评估组织质量和患者的临床结果。方法：骨软骨活检取自体软骨细胞植入(ACI)修复软骨缺损的患者(14例)，术后9~65个月。组织学采用OsScore评分，IHC检测I型和II型胶原的存在，FT-IRIS检测蛋白多糖(PG)的分布和胶原的质量参数。通过Lysholm评分评估患者的临床结果。结果：79%的患者Lysholm评分有改善。免疫组织化学染色显示，所有标本均有I型和II型胶原存在，深部II型胶原含量较多。II型胶原免疫染色的数量和位置与Fr-IRIS衍生的相对PG含量和胶原螺旋完整性参数相关。此外，ACI后Lysholm评分的改善与OsScore、II型胶原(IHC评分)和FT-IRIS确定的参数呈正相关。Lysholm评分的改善与OsScore、IHC面积评分或FT-IRIS参数之间的回归模型均达到显著水平(p<；0.01)。然而，包含OsScore和IHC评分参数的FT-IRIS模型并没有显著改善。结论：软骨修复组织的FT-IRIS派生分子参数与患者临床结果之间的相关性为将该方法转换到临床环境中以辅助软骨疾病的管理和治疗奠定了基础。(C)2012年国际骨性关节炎研究会。爱思唯尔有限公司出版。保留所有权利。
Objective: To investigate whether Fourier transform infrared imaging spectroscopy (FT-IRIS), a modality based on molecular vibrations, is a viable alternative to histology and immunohistochemistry (IHC) for assessment of tissue quality and patient clinical outcome.Methods: Osteochondral biopsies were obtained from patients (9-65 months post-surgery) who underwent an autologous chondrocyte implantation (ACI) procedure to repair a cartilage defect (N = 14). The repair tissue was evaluated histologically by OsScore grading, for the presence of types I and II collagen by IHC, and for proteoglycan (PG) distribution and collagen quality parameters by FT-IRIS. Patient clinical outcome was assessed by the Lysholm score.Results: Improvement in Lysholm score occurred in 79% of patients. IHC staining showed the presence of types I and II collagen in all samples, with a greater amount of collagen type II in the deep zone. The amount and location of immunostaining for type II collagen correlated to the Fr-IRIS-derived parameters of relative PG content and collagen helical integrity. In addition, the improvement in Lysholm score post-ACI correlated positively with the OsScore, type II collagen (IHC score) and FT-IRIS-determined parameters. Regression models for the relation between improvement in Lysholm score and either OsScore, IHC area score or the FT-IRIS parameters all reached significance (p < 0.01). However, the FT-IRIS model was not significantly improved with inclusion of the OsScore and IHC score parameters.Conclusion: Demonstration of the correlation between FT-IRIS-derived molecular parameters of cartilage repair tissue and patient clinical outcome lays the groundwork for translation of this methodology to the clinical environment to aid in the management of cartilage disorders and their treatment. (c) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.